| Title |
Characterization of the Nicotiana tabacum L. genome by molecular cytogenetics
|
|---|---|
| Published in |
Molecular Genetics and Genomics, August 1993
|
| DOI | 10.1007/bf00277053 |
| Pubmed ID | |
| Authors |
Ann Kenton, Alex S. Parokonny, Yuri Y. Gleba, Michael D. Bennett |
| Abstract |
Nicotiana tabacum (2n = 48) is a natural amphidiploid with component genomes S and T. We used non-radioactive in situ hybridization to provide physical chromosome markers for N. tabacum, and to determine the extant species most similar to the S and T genomes. Chromosomes of the S genome hybridized strongly to biotinylated total DNA from N. sylvestris, and showed the same physical localization of a tandemly repeated DNA sequence, HRS 60.1, confirming the close relationship between the S genome and N. sylvestris. Results of dot blot and in situ hybridizations of N. tabacum DNA to biotinylated total genomic DNA from N. tomentosiformis and N. otophora suggested that the T genome may derive from an introgressive hybrid between these two species. Moreover, a comparison of nucleolus-organizing chromosomes revealed that the nucleolus organizer region (NOR) most strongly expressed in N. tabacum had a very similar counterpart in N. otophora. Three different N. tabacum genotypes each had up to 9 homozygous translocations between chromosomes of the S and T genomes. Such translocations, which were either unilateral or reciprocal, demonstrate that intergenomic transfer of DNA has occurred in the amphidiploid, possibly accounting for some results of previous genetic and molecular analyses. Molecular cytogenetics of N. tabacum has identified new chromosome markers, providing a basis for physical gene mapping and showing that the amphidiploid genome has diverged structurally from its ancestral components. |
Mendeley readers
The data shown below were compiled from readership statistics for 71 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Belgium | 1 | 1% |
| Unknown | 70 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 13 | 18% |
| Researcher | 11 | 15% |
| Student > Bachelor | 10 | 14% |
| Student > Ph. D. Student | 8 | 11% |
| Professor | 5 | 7% |
| Other | 15 | 21% |
| Unknown | 9 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 36 | 51% |
| Biochemistry, Genetics and Molecular Biology | 17 | 24% |
| Medicine and Dentistry | 3 | 4% |
| Social Sciences | 2 | 3% |
| Mathematics | 1 | 1% |
| Other | 2 | 3% |
| Unknown | 10 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 February 2022.
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#8,534,528
of 25,371,288 outputs
Outputs from Molecular Genetics and Genomics
#920
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#5,709
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Outputs of similar age from Molecular Genetics and Genomics
#7
of 21 outputs
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