Title |
miR-137 inhibits melanoma cell proliferation through downregulation of GLO1
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Published in |
Science China Life Sciences, January 2018
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DOI | 10.1007/s11427-017-9138-9 |
Pubmed ID | |
Authors |
Na Lv, Shuai Hao, Chonglin Luo, Alia Abukiwan, Ying Hao, Fei Gai, Weiwei Huang, Lingyun Huang, Xueyuan Xiao, Stefan B. Eichmüller, Dacheng He |
Abstract |
Late-stage melanoma is refractory to current therapies. MicroRNAs (miRNAs) can modulate many physiological and pathological processes of melanoma. Studies have demonstrated that miR-137 acts as a tumor suppressor by inhibiting the proliferation of melanoma cells through targeting multiple mRNAs. The glyoxalase system member glyoxalase 1 (GLO1) is the principal scavenging enzyme of methylglyoxal (MG), a toxic byproduct of glycolysis. Using 35S in vivo/vitro labelling analysis for dynamic proteomics (SiLAD), we found that miR-137 downregulated the expression of GLO1 in melanoma cells. Bioinformatics analysis predicted that GLO1 is a direct target of miR-137. This was validated by dual luciferase reporter assay. Quantitative RT-PCR (qRT-PCR) and western blot analysis indicated that miR-137 could decrease endogenous GLO1 expression. Furthermore, siRNA targeting of GLO1 mimicked inhibition of melanoma cell proliferation caused by miR-137 overexpression. Re-expression of GLO1 was able to restore miR-137-mediated suppression of melanoma cell proliferation. Therefore, these results suggest that miR-137 inhibits the proliferation of melanoma cells by targeting GLO1. |
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