| Title |
Mycobacteria: Bugs and bugbears (Two steps forward and one step back)
|
|---|---|
| Published in |
Molecular Biotechnology, January 1999
|
| DOI | 10.1385/mb:13:3:191 |
| Pubmed ID | |
| Authors |
Tanya Parish, Neil G. Stoker |
| Abstract |
The use of molecular techniques to study the mycobacteria has advanced greatly since the first genomic libraries of Mycobacterium tuberculosis and M. leprae were constructed in 1985. However, there are still pitfalls for the unwary. Most of the problems associated with the use of molecular techniques to study mycobacteria can be related to one of the following problems: slow growth rate causing problems with contamination; the formation of macroscopic clumps when grown in culture; resistance to standard chemical lysis procedures; the requirement for containment facilities for pathogenic species; the lack of suitable genetic vectors; and the problems of spontaneous antibiotic resistance. Despite these problems, considerable progress has been made and standard techniques have been developed for the preparation of protein, nucleic acids (DNA and RNA) and cell wall components, chemical and transposon mutagenesis and gene replacement methods, the use of reporter genes and expression vectors, and improved detection and drug sensitivity testing. |
Mendeley readers
The data shown below were compiled from readership statistics for 79 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 1% |
| Canada | 1 | 1% |
| Unknown | 77 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 17 | 22% |
| Researcher | 9 | 11% |
| Student > Master | 9 | 11% |
| Student > Ph. D. Student | 8 | 10% |
| Student > Doctoral Student | 6 | 8% |
| Other | 13 | 16% |
| Unknown | 17 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 16 | 20% |
| Medicine and Dentistry | 11 | 14% |
| Agricultural and Biological Sciences | 10 | 13% |
| Chemistry | 8 | 10% |
| Environmental Science | 3 | 4% |
| Other | 8 | 10% |
| Unknown | 23 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 March 2021.
All research outputs
#5,446,210
of 25,373,627 outputs
Outputs from Molecular Biotechnology
#140
of 1,130 outputs
Outputs of similar age
#10,337
of 109,585 outputs
Outputs of similar age from Molecular Biotechnology
#4
of 27 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,130 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done well, scoring higher than 77% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 109,585 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 83% of its contemporaries.
We're also able to compare this research output to 27 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.