| Title |
Assessment of wavelength-dependent parameters of photosynthetic electron transport with a new type of multi-color PAM chlorophyll fluorometer
|
|---|---|
| Published in |
Photosynthesis Research, June 2012
|
| DOI | 10.1007/s11120-012-9758-1 |
| Pubmed ID | |
| Authors |
Ulrich Schreiber, Christof Klughammer, Jörg Kolbowski |
| Abstract |
Technical features of a novel multi-color pulse amplitude modulation (PAM) chlorophyll fluorometer as well as the applied methodology and some typical examples of its practical application with suspensions of Chlorella vulgaris and Synechocystis PCC 6803 are presented. The multi-color PAM provides six colors of pulse-modulated measuring light (peak-wavelengths at 400, 440, 480, 540, 590, and 625 nm) and six colors of actinic light (AL), peaking at 440, 480, 540, 590, 625 and 420-640 nm (white). The AL can be used for continuous illumination, maximal intensity single-turnover pulses, high intensity multiple-turnover pulses, and saturation pulses. In addition, far-red light (peaking at 725 nm) is provided for preferential excitation of PS I. Analysis of the fast fluorescence rise kinetics in saturating light allows determination of the wavelength- and sample-specific functional absorption cross section of PS II, Sigma(II)(λ), with which the PS II turnover rate at a given incident photosynthetically active radiation (PAR) can be calculated. Sigma(II)(λ) is defined for a quasi-dark reference state, thus differing from σ(PSII) used in limnology and oceanography. Vastly different light response curves for Chlorella are obtained with light of different colors, when the usual PAR-scale is used. Based on Sigma(II)(λ) the PAR, in units of μmol quanta/(m(2) s), can be converted into PAR(II) (in units of PS II effective quanta/s) and a fluorescence-based electron transport rate ETR(II) = PAR(II) · Y(II)/Y(II)(max) can be defined. ETR(II) in contrast to rel.ETR qualifies for quantifying the absolute rate of electron transport in optically thin suspensions of unicellular algae and cyanobacteria. Plots of ETR(II) versus PAR(II) for Chlorella are almost identical using either 440 or 625 nm light. Photoinhibition data are presented suggesting that a lower value of ETR(II)(max) with 440 nm possibly reflects photodamage via absorption by the Mn-cluster of the oxygen-evolving complex. |
Mendeley readers
The data shown below were compiled from readership statistics for 186 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | <1% |
| Australia | 1 | <1% |
| Brazil | 1 | <1% |
| South Africa | 1 | <1% |
| Nigeria | 1 | <1% |
| Russia | 1 | <1% |
| United States | 1 | <1% |
| Unknown | 179 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 47 | 25% |
| Researcher | 32 | 17% |
| Student > Master | 21 | 11% |
| Student > Bachelor | 18 | 10% |
| Student > Doctoral Student | 14 | 8% |
| Other | 25 | 13% |
| Unknown | 29 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 75 | 40% |
| Environmental Science | 25 | 13% |
| Biochemistry, Genetics and Molecular Biology | 14 | 8% |
| Earth and Planetary Sciences | 9 | 5% |
| Physics and Astronomy | 5 | 3% |
| Other | 19 | 10% |
| Unknown | 39 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 August 2019.
All research outputs
#7,451,584
of 22,780,967 outputs
Outputs from Photosynthesis Research
#194
of 769 outputs
Outputs of similar age
#54,764
of 164,546 outputs
Outputs of similar age from Photosynthesis Research
#1
of 3 outputs
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So far Altmetric has tracked 769 research outputs from this source. They receive a mean Attention Score of 3.7. This one is in the 42nd percentile – i.e., 42% of its peers scored the same or lower than it.
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