| Title |
Evaluation of the Efficiency of Lytic Mycobacteriophage D29 on the Model of M. tuberculosis-Infected Macrophage RAW 264 Cell Line
|
|---|---|
| Published in |
Bulletin of Experimental Biology and Medicine, January 2018
|
| DOI | 10.1007/s10517-018-3986-0 |
| Pubmed ID | |
| Authors |
M. B. Lapenkova, N. S. Smirnova, P. N. Rutkevich, M. A. Vladimirsky |
| Abstract |
Culture of mouse macrophages (RAW 264.7 ATCC strain) in wells of a 6-well plate was infected with M. tuberculosis in proportion of 15 mycobacteria per one macrophage and then treated with a lytic strain of mycobacteriophage D29. Antibacterial efficacy of mycobacteriophages was studied using D29 phage (activity 108 plaque-forming units/ml) previously purified by ion exchange chromatography. After single and double 24-h treatment, the lysed cultures of macrophages were inoculated onto Middlebrook 7H10 agar medium. The number of mycobacterial colonies in control and test wells (at least 3 wells in each group) was 300.178±12.500 and 36.0±5.4, respectively (p<0.01). |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 2 | 67% |
| United Kingdom | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 26 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 7 | 27% |
| Student > Ph. D. Student | 4 | 15% |
| Researcher | 3 | 12% |
| Student > Postgraduate | 3 | 12% |
| Professor > Associate Professor | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 7 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 27% |
| Agricultural and Biological Sciences | 5 | 19% |
| Chemistry | 2 | 8% |
| Medicine and Dentistry | 2 | 8% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
| Other | 2 | 8% |
| Unknown | 7 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 August 2018.
All research outputs
#19,015,393
of 24,217,893 outputs
Outputs from Bulletin of Experimental Biology and Medicine
#726
of 1,357 outputs
Outputs of similar age
#321,033
of 450,115 outputs
Outputs of similar age from Bulletin of Experimental Biology and Medicine
#4
of 11 outputs
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So far Altmetric has tracked 1,357 research outputs from this source. They receive a mean Attention Score of 2.3. This one is in the 43rd percentile – i.e., 43% of its peers scored the same or lower than it.
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