| Title |
Advanced glycation end products as a source of artifacts in immunoenzymatic methods
|
|---|---|
| Published in |
Glycoconjugate Journal, January 2018
|
| DOI | 10.1007/s10719-017-9805-4 |
| Pubmed ID | |
| Authors |
Aleksandra Kuzan, A. Chwiłkowska, K. Maksymowicz, A. Bronowicka-Szydełko, K. Stach, C. Pezowicz, A. Gamian |
| Abstract |
The most abundant proteins in the arteries are those of extracellular matrix, ie. collagen and elastin. Due to their long half-lifes these proteins have an increased chance to undergo glycation. The aim of this study was to determine relationship between the content of the main extracellular matrix proteins and the advanced glycation end products (AGEs) in arteries. In this study 103 fragments of aorta were analyzed by ELISA and immunobloting for the content of collagens type I, III and IV and elastin and the content of advanced glycation end-products (AGE). A negative correlation between the content of collagens type III and IV and AGE (r = -0,258, p = 0,0122, and a weak negative correlation between collagen type III and age of the sample donor (r = 0,218, p = 0,0262) were demonstrated. This result comes as a surprise and it contradicts an intuitive assumption that with more glycation substrate, i.e. matrix proteins, more AGE products are expected. We have concluded that the results of the ELISA tests must have been influenced by the glycation. As a consequence, either modified protein molecules were not being recognized by the antibodies, or the glycation, and formation of crosslinks have blocked access of the antibodies to the antigen. It will conceal the effect of the linear dependence between the result (absorbance/densitometry) from the quantity of protein to which the antibody is directed. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 25% |
| Japan | 1 | 25% |
| Unknown | 2 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Science communicators (journalists, bloggers, editors) | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 20 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor > Associate Professor | 4 | 20% |
| Other | 2 | 10% |
| Student > Bachelor | 2 | 10% |
| Researcher | 2 | 10% |
| Student > Ph. D. Student | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 7 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Engineering | 5 | 25% |
| Medicine and Dentistry | 3 | 15% |
| Agricultural and Biological Sciences | 2 | 10% |
| Biochemistry, Genetics and Molecular Biology | 1 | 5% |
| Unknown | 9 | 45% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 September 2021.
All research outputs
#16,053,755
of 25,382,440 outputs
Outputs from Glycoconjugate Journal
#662
of 929 outputs
Outputs of similar age
#255,406
of 449,622 outputs
Outputs of similar age from Glycoconjugate Journal
#2
of 7 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. This one is in the 34th percentile – i.e., 34% of other outputs scored the same or lower than it.
So far Altmetric has tracked 929 research outputs from this source. They receive a mean Attention Score of 3.8. This one is in the 26th percentile – i.e., 26% of its peers scored the same or lower than it.
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We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one. This one has scored higher than 5 of them.