Title |
Structure, mechanism and catalytic duality of thiamine-dependent enzymes
|
---|---|
Published in |
Cellular and Molecular Life Sciences, February 2007
|
DOI | 10.1007/s00018-007-6423-5 |
Pubmed ID | |
Authors |
R. A. W. Frank, F. J. Leeper, B. F. Luisi |
Abstract |
Thiamine is an essential cofactor that is required for processes of general metabolism amongst all organisms, and it is likely to have played a role in the earliest stages of the evolution of life. Here, we review from a structural perspective the enzymatic mechanisms that involve this cofactor. We explore asymmetry within homodimeric thiamine diphosphate (ThDP)-dependent enzyme structures and discuss how this may be correlated with the kinetic properties of half-of-the-sites reactivity, and negative cooperativity. It is likely these structural and kinetic hallmarks may arise through reciprocal coupling of active sites. This mode of communication between distant active sites is not unique to ThDP-dependent enzymes, but is widespread in other classes of oligomeric enzyme. Thus, it appears likely to be a general phenomenon reflecting a powerful mechanism of accelerating the rate of a chemical pathway. Finally, we speculate on the early evolutionary history of the cofactor and its ancient association with protein and RNA. |
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France | 1 | <1% |
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Unknown | 171 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 36 | 20% |
Student > Master | 31 | 17% |
Student > Bachelor | 23 | 13% |
Researcher | 23 | 13% |
Student > Postgraduate | 13 | 7% |
Other | 31 | 17% |
Unknown | 25 | 14% |
Readers by discipline | Count | As % |
---|---|---|
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Biochemistry, Genetics and Molecular Biology | 31 | 17% |
Chemistry | 29 | 16% |
Medicine and Dentistry | 14 | 8% |
Engineering | 10 | 5% |
Other | 18 | 10% |
Unknown | 27 | 15% |