| Title |
DNA damage after acute exposure of mice skin to physiological doses of UVB and UVA light
|
|---|---|
| Published in |
Archives of Dermatological Research, January 2012
|
| DOI | 10.1007/s00403-012-1212-x |
| Pubmed ID | |
| Authors |
Alena Rajnochová Svobodová, Adéla Galandáková, Jarmila Šianská, Dalibor Doležal, Radka Lichnovská, Jitka Ulrichová, Jitka Vostálová |
| Abstract |
Solar ultraviolet (UV) radiation is an important risk factor in skin carcinogenesis. This has been attributed mainly to the UVB waveband because the high-energetic photons are capable of interacting with DNA and inducing DNA damage. Recently, UVA light has also gained increasing interest in relation to DNA alteration. Although UVA photons are less energetic than UVB, they comprise a major fraction of sunlight UV radiation and penetrate deep into the skin. The study was carried out to compare the acute effects of UVA and UVB light on SKH-1 mice in relation to DNA damage and associated parameters. Mice were exposed to UVA (10 and 20 J/cm(2)) or UVB (200 and 800 mJ/cm(2)) radiation. The number of DNA single-strand breaks (SSB) in lymphocytes, amount of phosphorylated histone H2AX (gamma-H2AX) and apoptosis or DNA fragmentation (TUNEL-positive cells) in skin sections and level of gamma-H2AX, activated caspase-3 and phosphorylated p53 in skin were evaluated after 4 and 24 h. SSB analyzed by alkaline comet assay were found to be 4 and 24 h following UVB and UVA treatment, respectively. TUNEL and gamma-H2AX-positive cell were observed only in UVB exposed animals at both time intervals. The level of activated caspase-3 and phospho-p53 was increased 24 h after UVA and UVB radiation and was more apparent in UVB treated mice. The results indicate that the mechanism of DNA damage caused by acute UVA exposure includes formation of SSB (oxidative damage), but not double-strand breaks. |
Mendeley readers
The data shown below were compiled from readership statistics for 102 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Belgium | 1 | <1% |
| Slovenia | 1 | <1% |
| Unknown | 100 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 23 | 23% |
| Student > Master | 16 | 16% |
| Student > Bachelor | 11 | 11% |
| Other | 8 | 8% |
| Researcher | 8 | 8% |
| Other | 13 | 13% |
| Unknown | 23 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 22 | 22% |
| Biochemistry, Genetics and Molecular Biology | 18 | 18% |
| Medicine and Dentistry | 14 | 14% |
| Chemistry | 6 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 5 | 5% |
| Other | 11 | 11% |
| Unknown | 26 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 May 2023.
All research outputs
#7,451,584
of 22,780,967 outputs
Outputs from Archives of Dermatological Research
#331
of 1,325 outputs
Outputs of similar age
#71,695
of 246,490 outputs
Outputs of similar age from Archives of Dermatological Research
#2
of 7 outputs
Altmetric has tracked 22,780,967 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,325 research outputs from this source. They receive a mean Attention Score of 4.8. This one is in the 36th percentile – i.e., 36% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 246,490 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 43rd percentile – i.e., 43% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one. This one has scored higher than 5 of them.