| Title |
Antimicrobial peptide gene BdPho responds to peptidoglycan infection and mating stimulation in oriental fruit fly, Bactrocera dorsalis (Hendel)
|
|---|---|
| Published in |
AMB Express, January 2018
|
| DOI | 10.1186/s13568-017-0533-8 |
| Pubmed ID | |
| Authors |
Shi-Huo Liu, Hong-Fei Li, Yang Yang, Dong Wei, Hong-Bo Jiang, Wei Dou, Guo-Rui Yuan, Jin-Jun Wang |
| Abstract |
Phormicins belong to defensin family, which are important antimicrobial peptides (AMPs) in insects. These AMPs are inducible upon challenging by immune triggers. In the present study, we identified the cDNA of a phormicin gene (BdPho) in the oriental fruit fly, Bactrocera dorsalis (Hendel), a ruinous agricultural pest causing great economic losses to fruits and vegetables. The cDNA of BdPho contains a 282 bp open reading frame encoding 93 amino acid residues, and the predicted molecular weight and isoelectric point of BdPho peptide were 9.83 kDa and 7.54, respectively. Quantitative real-time PCR analyses showed that the transcription level of BdPho was the highest in adult during different developmental stages and was the highest in abdomen among adult tagmata. Moreover, BdPho was highly expressed in fat body among different tissues, both in female and male adult. The mRNA level of BdPho was significantly up-regulated to 7.46- and 14.53-fold at 3 and 6 h after the insects were challenged with peptidoglycans from Escherichia coli (PGN-EB), respectively, suggesting its antimicrobial activity against Gram-negative microorganisms. Furthermore, the expression level of BdPho was significantly up-regulated to 3.83-fold after mating, suggesting that female adults might enhance their immunity by up-regulating the expression level of BdPho during mating. These results firstly describe the basic properties of the phormicin gene from B. dorsalis, and lay the foundation for investigating functional properties of AMPs and exploring the molecular mechanisms in the immune system. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 2 | 20% |
| Librarian | 1 | 10% |
| Student > Doctoral Student | 1 | 10% |
| Student > Bachelor | 1 | 10% |
| Student > Ph. D. Student | 1 | 10% |
| Other | 2 | 20% |
| Unknown | 2 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 1 | 10% |
| Arts and Humanities | 1 | 10% |
| Biochemistry, Genetics and Molecular Biology | 1 | 10% |
| Agricultural and Biological Sciences | 1 | 10% |
| Chemistry | 1 | 10% |
| Other | 1 | 10% |
| Unknown | 4 | 40% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 January 2018.
All research outputs
#15,488,947
of 23,016,919 outputs
Outputs from AMB Express
#447
of 1,241 outputs
Outputs of similar age
#270,810
of 443,312 outputs
Outputs of similar age from AMB Express
#14
of 41 outputs
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