Title |
Development of a psilocin immunoassay for serum and blood samples
|
---|---|
Published in |
International Journal of Legal Medicine, August 2004
|
DOI | 10.1007/s00414-004-0469-9 |
Pubmed ID | |
Authors |
C. Albers, H. Köhler, M. Lehr, B. Brinkmann, J. Beike |
Abstract |
After the immunisation of rabbits with a psilocin-specific immunogen, polyclonal antisera were obtained. With these antisera a competitive, heterogeneous radioimmunoassay for the detection of psilocin was developed. As tracer a derivative of psilocin was synthesised, which contained a tritiated CH(3) group. The antisera showed a specific reaction with psilocin. The cross-reactivity of structurally related endogenous substances like serotonin, tryptophan and tyrosine was below 0.01%. Also common drugs of abuse (Delta(9)-tetrahydrocannabinol, cocaine, morphine, amphetamine) showed negligible cross-reactivity (0.01-2%). Only tricyclic neuroleptics with a (dimethylamino)ethyl side-chain showed some cross-reactivity (20%). Spiked serum and blood samples were analysed with this new immunoassay and the results obtained were compared with the values measured with a validated GC-MS method. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 3% |
Unknown | 36 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 7 | 19% |
Student > Bachelor | 7 | 19% |
Student > Ph. D. Student | 4 | 11% |
Student > Doctoral Student | 3 | 8% |
Other | 3 | 8% |
Other | 4 | 11% |
Unknown | 9 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 7 | 19% |
Neuroscience | 5 | 14% |
Agricultural and Biological Sciences | 4 | 11% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 8% |
Biochemistry, Genetics and Molecular Biology | 2 | 5% |
Other | 5 | 14% |
Unknown | 11 | 30% |