| Title |
Genetically engineered avidins and streptavidins
|
|---|---|
| Published in |
Cellular and Molecular Life Sciences, November 2006
|
| DOI | 10.1007/s00018-006-6288-z |
| Pubmed ID | |
| Authors |
O. H. Laitinen, V. P. Hytönen, H. R. Nordlund, M. S. Kulomaa |
| Abstract |
Chicken avidin and bacterial streptavidin, (strept)avidin, are proteins widely utilized in a number of applications in life science, ranging from purification and labeling techniques to diagnostics, and from targeted drug delivery to nanotechnology. (Strept)avidin-biotin technology relies on the extremely tight and specific affinity between (strept)avidin and biotin (dissociation constant, K(d) approximately 10(-14)-10(-16) M). (Strept)avidins are also exceptionally stable proteins. To study their ligand binding and stability characteristics, the two proteins have been extensively modified both chemically and genetically. There are excellent accounts of this technology and chemically modified (strept)avidins, but no comprehensive reviews exist concerning genetically engineered (strept)avidins. To fill this gap, we here go through the genetically engineered (strept)avidins, summarizing how these constructs were designed and how they have improved our understanding of the structural and functional characteristics of these proteins, and the benefits they have provided for (strept)avidin-biotin technology. |
Mendeley readers
The data shown below were compiled from readership statistics for 203 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 5 | 2% |
| Germany | 4 | 2% |
| Canada | 3 | 1% |
| France | 1 | <1% |
| Australia | 1 | <1% |
| India | 1 | <1% |
| Switzerland | 1 | <1% |
| Japan | 1 | <1% |
| Sweden | 1 | <1% |
| Other | 0 | 0% |
| Unknown | 185 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 55 | 27% |
| Researcher | 32 | 16% |
| Student > Master | 29 | 14% |
| Student > Bachelor | 26 | 13% |
| Professor > Associate Professor | 10 | 5% |
| Other | 22 | 11% |
| Unknown | 29 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 53 | 26% |
| Biochemistry, Genetics and Molecular Biology | 38 | 19% |
| Chemistry | 36 | 18% |
| Engineering | 10 | 5% |
| Physics and Astronomy | 8 | 4% |
| Other | 22 | 11% |
| Unknown | 36 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 27. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 February 2023.
All research outputs
#1,310,807
of 23,794,258 outputs
Outputs from Cellular and Molecular Life Sciences
#112
of 4,151 outputs
Outputs of similar age
#2,196
of 70,719 outputs
Outputs of similar age from Cellular and Molecular Life Sciences
#1
of 33 outputs
Altmetric has tracked 23,794,258 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 94th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,151 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.0. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 70,719 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 96% of its contemporaries.
We're also able to compare this research output to 33 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 96% of its contemporaries.