Title |
Complete genome sequence and construction of an infectious full-length cDNA clone of a German isolate of celery mosaic virus
|
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Published in |
Archives of Virology, January 2018
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DOI | 10.1007/s00705-018-3705-1 |
Pubmed ID | |
Authors |
Hanna Rose, Edgar Maiss |
Abstract |
The complete genome sequence of a German isolate of celery mosaic virus (CeMV, a potyvirus) from Quedlinburg (DSMZ PV-1003) was determined (MF962880). This represents the second fully sequenced genome of this virus, along with a Californian isolate (HQ676607.1). The positive-sense single-stranded RNA is 10,000 nucleotides in length and shows the typical organization of potyviruses but has a shorter PIPO than CeMV California. In comparison to CeMV isolates from different origins, CeMV-Quedlinburg and isolates from the Netherlands (AF203531.1) and Aschersleben, Germany (AJ271087.1) show a NAG instead of DAG in the region of the coat protein responsible for aphid transmission. In this study the first infectious full-length clone of celery mosaic virus was obtained and the infectivity confirmed by Rhizobium radiobacter infiltration of Apium species. |
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Unknown | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 11 | 100% |
Demographic breakdown
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Student > Ph. D. Student | 5 | 45% |
Student > Bachelor | 1 | 9% |
Librarian | 1 | 9% |
Professor | 1 | 9% |
Researcher | 1 | 9% |
Other | 0 | 0% |
Unknown | 2 | 18% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 8 | 73% |
Medicine and Dentistry | 1 | 9% |
Unknown | 2 | 18% |