| Title |
Simultaneous pre-concentration and separation on simple paper-based analytical device for protein analysis
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, January 2018
|
| DOI | 10.1007/s00216-017-0809-5 |
| Pubmed ID | |
| Authors |
Ji-Cheng Niu, Ting Zhou, Li-Li Niu, Zhen-Sheng Xie, Fang Fang, Fu-Quan Yang, Zhi-Yong Wu |
| Abstract |
In this work, fast isoelectric focusing (IEF) was successfully implemented on an open paper fluidic channel for simultaneous concentration and separation of proteins from complex matrix. With this simple device, IEF can be finished in 10 min with a resolution of 0.03 pH units and concentration factor of 10, as estimated by color model proteins by smartphone-based colorimetric detection. Fast detection of albumin from human serum and glycated hemoglobin (HBA1c) from blood cell was demonstrated. In addition, off-line identification of the model proteins from the IEF fractions with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was also shown. This PAD IEF is potentially useful either for point of care test (POCT) or biomarker analysis as a cost-effective sample pretreatment method. |
X Demographics
The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 40% |
| Unknown | 3 | 60% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 40% |
| Members of the public | 2 | 40% |
| Science communicators (journalists, bloggers, editors) | 1 | 20% |
Mendeley readers
The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 44 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 16% |
| Student > Bachelor | 4 | 9% |
| Student > Master | 4 | 9% |
| Researcher | 4 | 9% |
| Student > Doctoral Student | 3 | 7% |
| Other | 8 | 18% |
| Unknown | 14 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 10 | 23% |
| Engineering | 6 | 14% |
| Medicine and Dentistry | 5 | 11% |
| Agricultural and Biological Sciences | 4 | 9% |
| Psychology | 2 | 5% |
| Other | 3 | 7% |
| Unknown | 14 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 February 2018.
All research outputs
#16,053,755
of 25,382,440 outputs
Outputs from Analytical & Bioanalytical Chemistry
#4,978
of 9,619 outputs
Outputs of similar age
#256,269
of 450,898 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#68
of 188 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. This one is in the 34th percentile – i.e., 34% of other outputs scored the same or lower than it.
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We're also able to compare this research output to 188 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 63% of its contemporaries.