Chapter title |
A Comprehensive Protocol Resource for Performing Pooled shRNA and CRISPR Screens
|
---|---|
Chapter number | 17 |
Book title |
Inflammation and Cancer
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7568-6_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7567-9, 978-1-4939-7568-6
|
Authors |
Leonie A. Cluse, Iva Nikolic, Deborah Knight, Piyush B. Madhamshettiwar, Jennii Luu, Karla J. Cowley, Timothy Semple, Gisela Mir Arnau, Jake Shortt, Ricky W. Johnstone, Kaylene J. Simpson, Cluse, Leonie A., Nikolic, Iva, Knight, Deborah, Madhamshettiwar, Piyush B., Luu, Jennii, Cowley, Karla J., Semple, Timothy, Arnau, Gisela Mir, Shortt, Jake, Johnstone, Ricky W., Simpson, Kaylene J. |
Abstract |
This chapter details a compendium of protocols that collectively enable the reader to perform a pooled shRNA and/or CRISPR screen-with methods to identify and validate positive controls and subsequent hits; establish a viral titer in the cell line of choice; create and screen libraries, sequence strategies, and bioinformatics resources to analyze outcomes. Collectively, this provides an overarching resource from the start to finish of a screening project, making this technology possible in all laboratories. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 32 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 8 | 25% |
Researcher | 8 | 25% |
Student > Master | 4 | 13% |
Student > Bachelor | 2 | 6% |
Other | 1 | 3% |
Other | 2 | 6% |
Unknown | 7 | 22% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 9 | 28% |
Agricultural and Biological Sciences | 9 | 28% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 6% |
Immunology and Microbiology | 2 | 6% |
Medicine and Dentistry | 2 | 6% |
Other | 1 | 3% |
Unknown | 7 | 22% |