| Title |
Cocaine, not morphine, causes the generation of reactive oxygen species and activation of NF-κB in transiently cotransfected heart cells
|
|---|---|
| Published in |
Cardiovascular Toxicology, January 2003
|
| DOI | 10.1385/ct:3:2:141 |
| Pubmed ID | |
| Authors |
Barbara Y. Hargrave, David A. Tiangco, Frank A. Lattanzio, Stephen J. Beebe |
| Abstract |
This study was designed to determine levels of NF-kappaB reporter gene activity and free radical generation in cultured striated myocytes (H9C2 cells) exposed to cocaine or morphine in the presence of free radical scavengers. Cells were transiently transfected with a NF-kappaB reporter gene and changes in luciferase activity were detected by bioluminescence. Using confocal microscopy and 2',7'-dichlorofluorescin diacetate, cocaine-induced or morphine-induced free radicals were quantified in H9C2 cells. Cocaine and morphine (0-1 x 10(-2) M) were tested separately. Cocaine but not morphine significantly activated NF-kappaB reporter gene activity in H9C2 cells. Overexpression of IkappaB inhibited NF-kappaB reporter activity at low (1 x 10(-4) M) but not high (1 x 10(-2) M) cocaine concentrations. Free radicals were generated in H9C2 cells stimulated with cocaine but not with morphine. The production of free radicals and NF-kappaB reporter gene activity could be blocked with N-acetylcysteine, glutathione, and, to a lesser extent, lipoic acid. The results suggest that cocaine induces free radical production, which leads to the activation of NF-kappaB signal transduction and possible inflammatory responses. |
Mendeley readers
The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 19 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 6 | 32% |
| Student > Master | 4 | 21% |
| Student > Doctoral Student | 3 | 16% |
| Professor | 2 | 11% |
| Student > Ph. D. Student | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 2 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 6 | 32% |
| Biochemistry, Genetics and Molecular Biology | 3 | 16% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 11% |
| Agricultural and Biological Sciences | 2 | 11% |
| Environmental Science | 1 | 5% |
| Other | 2 | 11% |
| Unknown | 3 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 January 2024.
All research outputs
#8,534,976
of 25,373,627 outputs
Outputs from Cardiovascular Toxicology
#59
of 320 outputs
Outputs of similar age
#33,675
of 136,759 outputs
Outputs of similar age from Cardiovascular Toxicology
#5
of 11 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 320 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one has gotten more attention than average, scoring higher than 64% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 136,759 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 11 others from the same source and published within six weeks on either side of this one. This one is in the 9th percentile – i.e., 9% of its contemporaries scored the same or lower than it.