| Title |
Purification and characterization of recombinant spider silk expressed in Escherichia coli
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, January 1998
|
| DOI | 10.1007/s002530051133 |
| Pubmed ID | |
| Authors |
S. Arcidiacono, C. Mello, D. Kaplan, S. Cheley, H. Bayley |
| Abstract |
A partial cDNA clone, from the 3' end of the dragline silk gene was isolated from Nephila clavipes major ampullate glands. This clone contains a 1.7-kb insert, consisting of a repetitive coding region of 1.4-kb and a 0.3-kb nonrepetitive coding region; 1.5-kb of the 1.7-kb fragment was cloned into Escherichia coli and a 43-kDa recombinant silk protein was expressed. Characterization of the purified protein by Western blot, amino acid composition analysis, and matrix-assisted laser desorption ionization/time-of-flight mass spectrometry confirms it to be spider dragline silk. |
Mendeley readers
The data shown below were compiled from readership statistics for 111 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 3% |
| United Kingdom | 1 | <1% |
| Germany | 1 | <1% |
| Japan | 1 | <1% |
| Russia | 1 | <1% |
| Unknown | 104 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 24 | 22% |
| Researcher | 19 | 17% |
| Student > Bachelor | 17 | 15% |
| Student > Master | 13 | 12% |
| Professor > Associate Professor | 8 | 7% |
| Other | 13 | 12% |
| Unknown | 17 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 24 | 22% |
| Biochemistry, Genetics and Molecular Biology | 23 | 21% |
| Chemistry | 16 | 14% |
| Materials Science | 11 | 10% |
| Engineering | 5 | 5% |
| Other | 9 | 8% |
| Unknown | 23 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 15. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 January 2021.
All research outputs
#2,330,307
of 25,374,917 outputs
Outputs from Applied Microbiology and Biotechnology
#185
of 8,290 outputs
Outputs of similar age
#2,161
of 94,806 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#1
of 21 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,290 research outputs from this source. They receive a mean Attention Score of 4.4. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 94,806 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 97% of its contemporaries.
We're also able to compare this research output to 21 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 85% of its contemporaries.