| Title |
The Bacterial Peptide Pheromone Plantaricin A Permeabilizes Cancerous, but not Normal, Rat Pituitary Cells and Differentiates between the Outer and Inner Membrane Leaflet
|
|---|---|
| Published in |
The Journal of Membrane Biology, July 2007
|
| DOI | 10.1007/s00232-007-9030-3 |
| Pubmed ID | |
| Authors |
Sverre L. Sand, Trude M. Haug, Jon Nissen-Meyer, Olav Sand |
| Abstract |
Plantaricin A (PlnA) is a 26-mer peptide pheromone with membrane-permeabilizing, strain-specific antibacterial activity, produced by Lactobacillus plantarum C11. We investigated the membrane-permeabilizing effects of PlnA on cultured cancerous and normal rat anterior pituitary cells using patch-clamp techniques and microfluorometry (fura-2). Cancerous cells displayed massive permeabilization within 5 s after exposure to 10-100 microM PlnA. The membrane depolarized to nearly 0 mV, and the membrane resistance decreased to a mere fraction of the initial value after less than 1 min. In outside-out membrane patches, 10 microM PlnA induced membrane currents reversing at 0 mV, which is compatible with an unspecific conductance increase. The D and L forms of the peptide had similar potency, indicating a nonchiral mechanism for the membrane-permeabilizing effect. Surprisingly, inside-out patches were insensitive to 1 mM PlnA. Primary cultures of normal rat anterior pituitary cells were also insensitive to the peptide. Thus, PlnA differentiates between plasma membranes and membrane leaflets. Microfluorometric recordings of [Ca(2+)](i) and cytosolic concentration of fluorochrome verified the rapid permeabilizing effect of PlnA on cancerous cells and the insensitivity of normal pituitary cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Chile | 1 | 3% |
| Italy | 1 | 3% |
| Unknown | 28 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 27% |
| Student > Ph. D. Student | 5 | 17% |
| Student > Postgraduate | 3 | 10% |
| Student > Bachelor | 2 | 7% |
| Student > Master | 2 | 7% |
| Other | 4 | 13% |
| Unknown | 6 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 30% |
| Biochemistry, Genetics and Molecular Biology | 6 | 20% |
| Immunology and Microbiology | 5 | 17% |
| Unspecified | 1 | 3% |
| Neuroscience | 1 | 3% |
| Other | 2 | 7% |
| Unknown | 6 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 December 2012.
All research outputs
#7,850,857
of 23,806,312 outputs
Outputs from The Journal of Membrane Biology
#169
of 803 outputs
Outputs of similar age
#20,382
of 55,346 outputs
Outputs of similar age from The Journal of Membrane Biology
#4
of 13 outputs
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