| Title |
Glioblastoma cells release factors that disrupt blood-brain barrier features
|
|---|---|
| Published in |
Acta Neuropathologica, January 2004
|
| DOI | 10.1007/s00401-003-0810-2 |
| Pubmed ID | |
| Authors |
Stefan W. Schneider, Thomas Ludwig, Lars Tatenhorst, Stephan Braune, Hans Oberleithner, Volker Senner, Werner Paulus |
| Abstract |
The blood-brain barrier (BBB), mediated by endothelial tight junctions, is defective in malignant gliomas such as glioblastoma, resulting in cerebral edema and contrast enhancement upon neuroradiological examination. The mechanisms underlying BBB breakdown are essentially unknown. Since non-neoplastic astrocytes are required to induce BBB features of cerebral endothelial cells, it is conceivable that malignant astrocytes have lost this ability due to dedifferentiation. Alternatively, glioma cells might actively degrade previously intact BBB tight junctions. To examine the latter hypothesis, we have employed a transepithelial electrical resistance breakdown assay using monolayers of the C7 subclone of Madin-Darby canine kidney (MDCK-C7) cells forming tight junctions similar to those of BBB endothelial cells. We found that glioblastoma primary cells co-cultured with the MDCK-C7 monolayer (without direct contact of the two cell types) resulted in marked breakdown of electrical resistance, whereas primary cultures derived from low-grade gliomas (fibrillary astrocytoma, oligoastrocytoma) showed delayed or no effects. These results suggest that malignant gliomas have acquired the ability to actively degrade tight junctions by secreting soluble factors, eventually leading to BBB disruption within invaded brain tissue. |
Mendeley readers
The data shown below were compiled from readership statistics for 150 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 2% |
| Israel | 1 | <1% |
| Brazil | 1 | <1% |
| Unknown | 145 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 38 | 25% |
| Student > Master | 27 | 18% |
| Researcher | 19 | 13% |
| Student > Doctoral Student | 8 | 5% |
| Student > Bachelor | 8 | 5% |
| Other | 22 | 15% |
| Unknown | 28 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 24 | 16% |
| Biochemistry, Genetics and Molecular Biology | 23 | 15% |
| Medicine and Dentistry | 22 | 15% |
| Neuroscience | 12 | 8% |
| Engineering | 8 | 5% |
| Other | 26 | 17% |
| Unknown | 35 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 November 2023.
All research outputs
#3,799,086
of 25,374,917 outputs
Outputs from Acta Neuropathologica
#962
of 2,527 outputs
Outputs of similar age
#10,290
of 147,249 outputs
Outputs of similar age from Acta Neuropathologica
#1
of 4 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,527 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 16.8. This one has gotten more attention than average, scoring higher than 60% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 147,249 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 4 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them