| Title |
Transfer of paternal mitochondrial DNA during fertilization of honeybee (Apis mellifera L.) eggs
|
|---|---|
| Published in |
Current Genetics, December 1993
|
| DOI | 10.1007/bf00351719 |
| Pubmed ID | |
| Authors |
Michael S. Meusel, Robin F. A. Moritz |
| Abstract |
Strict maternal inheritance of mitochondrial (mt) DNA is believed to be the rule in most eukaryotic organisms because of exclusion of paternal mitochondria from the egg cytoplasm during fertilization. In honeybees, polyspermic fertilization occurs, and many spermatozoa, including their mitochondria-rich flagellum, can completely penetrate the egg, thus allowing for a possibly high paternal leakage. In order to identify paternal mtDNA in honeybee eggs, restriction fragment length polymorphisms (RFLP) of different subspecies were used. Total DNA extracts of different developmental stages of an Apis mellifera carnica x Apis mellifera capensis hybrid brood were tested with a radioactively-labelled diagnostic mtDNA probe. Densitograms of autoradiographs indicated that the male contribution represents up to 27% of the total mitochondrial DNA in the fertilized eggs 12 h after oviposition. In subsequent developmental stages the portion of paternal mtDNA slowly decreased until hatching of the larvae when only traces were found. Although rapid disintegration of paternal mtDNA does not occur, the initially high paternal mitochondrial contribution is not maintained in the adult animal. |
Mendeley readers
The data shown below were compiled from readership statistics for 72 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 1% |
| Netherlands | 1 | 1% |
| Ireland | 1 | 1% |
| Sweden | 1 | 1% |
| United States | 1 | 1% |
| Unknown | 67 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 17 | 24% |
| Student > Ph. D. Student | 11 | 15% |
| Student > Postgraduate | 8 | 11% |
| Student > Master | 7 | 10% |
| Student > Bachelor | 5 | 7% |
| Other | 14 | 19% |
| Unknown | 10 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 31 | 43% |
| Biochemistry, Genetics and Molecular Biology | 10 | 14% |
| Environmental Science | 4 | 6% |
| Physics and Astronomy | 2 | 3% |
| Engineering | 2 | 3% |
| Other | 9 | 13% |
| Unknown | 14 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 August 2021.
All research outputs
#7,451,584
of 22,780,967 outputs
Outputs from Current Genetics
#329
of 1,203 outputs
Outputs of similar age
#14,265
of 70,712 outputs
Outputs of similar age from Current Genetics
#8
of 20 outputs
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