| Title |
Dependence of H2O2 Formation by Rat Heart Mitochondria on Substrate Availability and Donor Age
|
|---|---|
| Published in |
Journal of Bioenergetics and Biomembranes, February 1997
|
| DOI | 10.1023/a:1022420007908 |
| Pubmed ID | |
| Authors |
Richard G. Hansford, Barbara A. Hogue, Vida Mildaziene |
| Abstract |
We have examined the substrate specificity and inhibitor sensitivity of H2O2 formation by rat heart mitochondria. Active H2O2 production requires both a high fractional reduction of Complex I (indexed by NADH/NAD+ + NADH ratio) and a high membrane potential, delta psi. These conditions are achieved with supraphysiological concentrations of succinate. With physiological concentrations of NAD-linked substrates, rates of H2O2 formation are much lower (less than 0.1% of respiratory chain electron flux) but may be stimulated by the Complex III inhibitor antimycin A, but not by myxothiazol. Addition of Mn2+ to give 10 nmol/mg of mitochondrial protein enhances H2O2 production with all substrate combinations, possibly by repleting mitochondrial superoxide dismutase with this cation. Contrary to previously published work, no increased activity of H2O2 production was found with heart mitochondria from senescent (24 month) rats, relative to young adults (6 month). |
Mendeley readers
The data shown below were compiled from readership statistics for 90 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 1% |
| Serbia | 1 | 1% |
| Singapore | 1 | 1% |
| Unknown | 87 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 26 | 29% |
| Researcher | 13 | 14% |
| Student > Bachelor | 11 | 12% |
| Student > Master | 7 | 8% |
| Professor > Associate Professor | 5 | 6% |
| Other | 11 | 12% |
| Unknown | 17 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 32 | 36% |
| Biochemistry, Genetics and Molecular Biology | 22 | 24% |
| Medicine and Dentistry | 8 | 9% |
| Neuroscience | 2 | 2% |
| Sports and Recreations | 2 | 2% |
| Other | 5 | 6% |
| Unknown | 19 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 June 2016.
All research outputs
#8,535,472
of 25,374,647 outputs
Outputs from Journal of Bioenergetics and Biomembranes
#115
of 491 outputs
Outputs of similar age
#20,412
of 93,677 outputs
Outputs of similar age from Journal of Bioenergetics and Biomembranes
#1
of 1 outputs
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So far Altmetric has tracked 491 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
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