Title |
Isoprene synthesis protects transgenic tobacco plants from oxidative stress
|
---|---|
Published in |
Plant, Cell & Environment, April 2009
|
DOI | 10.1111/j.1365-3040.2009.01946.x |
Pubmed ID | |
Authors |
CLAUDIA E. VICKERS, MALCOLM POSSELL, CRISTIAN I. COJOCARIU, VIOLETA B. VELIKOVA, JULLADA LAOTHAWORNKITKUL, ANNETTE RYAN, PHILIP M. MULLINEAUX, C. NICHOLAS HEWITT |
Abstract |
Isoprene emission represents a significant loss of carbon to those plant species that synthesize this highly volatile and reactive compound. As a tool for studying the role of isoprene in plant physiology and biochemistry, we developed transgenic tobacco plants capable of emitting isoprene in a similar manner to and at rates comparable to a naturally emitting species. Thermotolerance of photosynthesis against transient high-temperature episodes could only be observed in lines emitting high levels of isoprene; the effect was very mild and could only be identified over repetitive stress events. However, isoprene-emitting plants were highly resistant to ozone-induced oxidative damage compared with their non-emitting azygous controls. In ozone-treated plants, accumulation of toxic reactive oxygen species (ROS) was inhibited, and antioxidant levels were higher. Isoprene-emitting plants showed remarkably decreased foliar damage and higher rates of photosynthesis compared to non-emitting plants immediately following oxidative stress events. An inhibition of hydrogen peroxide accumulation in isoprene-emitting plants may stall the programmed cell death response which would otherwise lead to foliar necrosis. These results demonstrate that endogenously produced isoprene provides protection from oxidative damage. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Italy | 2 | 1% |
United States | 2 | 1% |
Germany | 1 | <1% |
Australia | 1 | <1% |
Netherlands | 1 | <1% |
China | 1 | <1% |
United Kingdom | 1 | <1% |
Unknown | 139 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 33 | 22% |
Student > Ph. D. Student | 30 | 20% |
Student > Master | 13 | 9% |
Student > Doctoral Student | 11 | 7% |
Professor | 9 | 6% |
Other | 31 | 21% |
Unknown | 21 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 66 | 45% |
Environmental Science | 19 | 13% |
Biochemistry, Genetics and Molecular Biology | 13 | 9% |
Chemistry | 8 | 5% |
Earth and Planetary Sciences | 4 | 3% |
Other | 9 | 6% |
Unknown | 29 | 20% |