| Title |
Haplotype analysis of the germacrene A synthase gene and association with cynaropicrin content and biological activities in Cynara cardunculus
|
|---|---|
| Published in |
Molecular Genetics and Genomics, November 2017
|
| DOI | 10.1007/s00438-017-1388-z |
| Pubmed ID | |
| Authors |
Ana Margarida Ferro, Patrícia Ramos, Ângela Guerra, Paula Parreira, Teresa Brás, Olinda Guerreiro, Eliana Jerónimo, Carmen Capel, Juan Capel, Fernando J. Yuste-Lisbona, Maria F. Duarte, Rafael Lozano, M. Margarida Oliveira, Sónia Gonçalves |
| Abstract |
Cynara cardunculus: L. represents a natural source of terpenic compounds, with the predominant molecule being cynaropicrin. Cynaropicrin is gaining interest since it has been correlated to anti-hyperlipidaemia, antispasmodic and cytotoxicity activity against leukocyte cancer cells. The objective of this work was to screen a collection of C. cardunculus, from different origins, for new allelic variants in germacrene A synthase (GAS) gene involved in the cynaropicrin biosynthesis and correlate them with improved cynaropicrin content and biological activities. Using high-resolution melting, nine haplotypes were identified. The putative impact of the identified allelic variants in GAS protein was evaluated by bioinformatic tools and polymorphisms that putatively lead to protein conformational changes were described. Additionally, cynaropicrin and main pentacyclic triterpenes contents, and antithrombin, antimicrobial and antiproliferative activities were also determined in C. cardunculus leaf lipophilic-derived extracts. In this work we identified allelic variants with putative impact on GAS protein, which are significantly associated with cynaropicrin content and antiproliferative activity. The results obtained suggest that the identified polymorphisms should be explored as putative genetic markers correlated with biological properties in Cynara cardunculus. |
Mendeley readers
The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 33 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 24% |
| Student > Master | 6 | 18% |
| Other | 2 | 6% |
| Student > Doctoral Student | 2 | 6% |
| Librarian | 2 | 6% |
| Other | 4 | 12% |
| Unknown | 9 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 21% |
| Arts and Humanities | 3 | 9% |
| Business, Management and Accounting | 2 | 6% |
| Biochemistry, Genetics and Molecular Biology | 2 | 6% |
| Medicine and Dentistry | 2 | 6% |
| Other | 5 | 15% |
| Unknown | 12 | 36% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 April 2022.
All research outputs
#8,264,793
of 25,382,440 outputs
Outputs from Molecular Genetics and Genomics
#905
of 3,321 outputs
Outputs of similar age
#118,669
of 318,891 outputs
Outputs of similar age from Molecular Genetics and Genomics
#4
of 31 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. This one has received more attention than most of these and is in the 66th percentile.
So far Altmetric has tracked 3,321 research outputs from this source. They receive a mean Attention Score of 4.0. This one has gotten more attention than average, scoring higher than 72% of its peers.
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We're also able to compare this research output to 31 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 87% of its contemporaries.