| Title |
NDH-1L interacts with ferredoxin via the subunit NdhS in Thermosynechococcus elongatus
|
|---|---|
| Published in |
Photosynthesis Research, January 2015
|
| DOI | 10.1007/s11120-015-0090-4 |
| Pubmed ID | |
| Authors |
Zhihui He, Fangfang Zheng, Yaozong Wu, Qinghua Li, Jing Lv, Pengcheng Fu, Hualing Mi |
| Abstract |
The large size complex of cyanobacterial NAD(P)H dehydrogenase (NDH-1) complex (NDH-1L) plays crucial role in a variety of bioenergetic reactions such as respiration and cyclic electron flow around photosystem I. Although the complex has been isolated and identified, its biochemical function still remains to be clarified. Here, we highly purified the NDH-1L complex from the cells of Thermosynechococcus elongatus by Ni(2+) affinity chromatography and size-exclusion chromatography. The purified NDH-1L complex has an apparent total molecular mass of approximately 500 kDa. 14 known subunits were identified by mass spectrometry and immunoblotting, including the NdhS subunit containing ferredoxin (Fd)-docking site domain. Surface plasmon resonance measurement demonstrates that the NDH-1L complex could bind to Fd with the binding constant (K D) of 59 µM. Yeast two-hybrid system assay further confirmed the interaction of Fd with NdhS and indicated that NdhH is involved in the interaction. Our results provide direct biochemical evidence that the cyanobacterial NDH-1 complex catalyzes the electron transport from reduced Fd to plastoquinone via NdhS and NdhH. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 2 | 4% |
| United Kingdom | 1 | 2% |
| Unknown | 42 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 18% |
| Student > Master | 8 | 18% |
| Student > Ph. D. Student | 6 | 13% |
| Student > Bachelor | 5 | 11% |
| Student > Doctoral Student | 3 | 7% |
| Other | 4 | 9% |
| Unknown | 11 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 17 | 38% |
| Agricultural and Biological Sciences | 12 | 27% |
| Chemical Engineering | 1 | 2% |
| Immunology and Microbiology | 1 | 2% |
| Chemistry | 1 | 2% |
| Other | 0 | 0% |
| Unknown | 13 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 January 2015.
All research outputs
#20,252,875
of 22,782,096 outputs
Outputs from Photosynthesis Research
#688
of 769 outputs
Outputs of similar age
#297,240
of 353,568 outputs
Outputs of similar age from Photosynthesis Research
#12
of 15 outputs
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