| Title |
In situ, accurate, surface-enhanced Raman scattering detection of cancer cell nucleus with synchronous location by an alkyne-labeled biomolecular probe
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, December 2017
|
| DOI | 10.1007/s00216-017-0761-4 |
| Pubmed ID | |
| Authors |
Jing Zhang, Lijia Liang, Xin Guan, Rong Deng, Huixin Qu, Dianshuai Huang, Shuping Xu, Chongyang Liang, Weiqing Xu |
| Abstract |
A surface-enhanced Raman scattering (SERS) method for in situ detection and analysis of the intranuclear biomolecular information of a cell has been developed based on a small, biocompatible, nuclear-targeting alkyne-tagged deoxyribonucleic acid (DNA) probe (5-ethynyl-2'-deoxyuridine, EDU) that can specially accumulate in the cell nucleus during DNA replications to precisely locate the nuclear region without disturbance in cell biological activities and functions. Since the specific alkyne group shows a Raman peak in the Raman-silent region of cells, it is an interior label to visualize the nuclear location synchronously in real time when measuring the SERS spectra of a cell. Because no fluorescent-labeled dyes were used for locating cell nuclei, this method is simple, nondestructive, non- photobleaching, and valuable for the in situ exploration of vital physiological processes with DNA participation in cell organelles. Graphical abstract A universal strategy was developed to accurately locate the nuclear region and obtain precise molecular information of cell nuclei by SERS. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Mexico | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 18 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 28% |
| Professor > Associate Professor | 2 | 11% |
| Student > Doctoral Student | 1 | 6% |
| Other | 1 | 6% |
| Researcher | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 7 | 39% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 5 | 28% |
| Biochemistry, Genetics and Molecular Biology | 2 | 11% |
| Chemical Engineering | 1 | 6% |
| Materials Science | 1 | 6% |
| Design | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 8 | 44% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 October 2019.
All research outputs
#17,242,285
of 25,382,440 outputs
Outputs from Analytical & Bioanalytical Chemistry
#5,585
of 9,619 outputs
Outputs of similar age
#279,048
of 446,025 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#69
of 162 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. This one is in the 31st percentile – i.e., 31% of other outputs scored the same or lower than it.
So far Altmetric has tracked 9,619 research outputs from this source. They receive a mean Attention Score of 3.1. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
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We're also able to compare this research output to 162 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.