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Quantitative Analysis of Herpesvirus Sequences from Normal Tissue and Fibropapillomas of Marine Turtles with Real-Time PCR

Overview of attention for article published in Virology, August 2001
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Title
Quantitative Analysis of Herpesvirus Sequences from Normal Tissue and Fibropapillomas of Marine Turtles with Real-Time PCR
Published in
Virology, August 2001
DOI 10.1006/viro.2001.1023
Pubmed ID
Authors

Sandra L. Quackenbush, Rufina N. Casey, Rebecca J. Murcek, Thomas A. Paul, Thierry M. Work, Colin J. Limpus, Anny Chaves, Leslie duToit, Javier Vasconcelos Perez, A.Alonso Aguirre, Terry R. Spraker, Julia A. Horrocks, Lotus A. Vermeer, George H. Balazs, James W. Casey

Abstract

Quantitative real-time PCR has been used to measure fibropapilloma-associated turtle herpesvirus (FPTHV) pol DNA loads in fibropapillomas, fibromas, and uninvolved tissues of green, loggerhead, and olive ridley turtles from Hawaii, Florida, Costa Rica, Australia, Mexico, and the West Indies. The viral DNA loads from tumors obtained from terminal animals were relatively homogeneous (range 2-20 copies/cell), whereas DNA copy numbers from biopsied tumors and skin of otherwise healthy turtles displayed a wide variation (range 0.001-170 copies/cell) and may reflect the stage of tumor development. FPTHV DNA loads in tumors were 2.5-4.5 logs higher than in uninvolved skin from the same animal regardless of geographic location, further implying a role for FPTHV in the etiology of fibropapillomatosis. Although FPTHV pol sequences amplified from tumors are highly related to each other, single signature amino acid substitutions distinguish the Australia/Hawaii, Mexico/Costa Rica, and Florida/Caribbean groups.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 120 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 4 3%
United States 3 3%
Australia 1 <1%
Greece 1 <1%
France 1 <1%
Unknown 110 92%

Demographic breakdown

Readers by professional status Count As %
Researcher 21 18%
Student > Bachelor 21 18%
Student > Master 20 17%
Student > Ph. D. Student 14 12%
Professor > Associate Professor 8 7%
Other 19 16%
Unknown 17 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 55 46%
Environmental Science 14 12%
Veterinary Science and Veterinary Medicine 10 8%
Biochemistry, Genetics and Molecular Biology 8 7%
Medicine and Dentistry 6 5%
Other 9 8%
Unknown 18 15%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 March 2014.
All research outputs
#8,534,976
of 25,374,647 outputs
Outputs from Virology
#3,591
of 9,498 outputs
Outputs of similar age
#13,857
of 40,372 outputs
Outputs of similar age from Virology
#23
of 63 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 9,498 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.6. This one is in the 17th percentile – i.e., 17% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 40,372 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 11th percentile – i.e., 11% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 63 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.