| Title |
Oxygen sensitivity of algal H2- production
|
|---|---|
| Published in |
Applied Biochemistry and Biotechnology, March 1997
|
| DOI | 10.1007/bf02920420 |
| Pubmed ID | |
| Authors |
Maria L. Ghirardi, Robert K. Togasaki, Michael Seibert |
| Abstract |
Photoproduction of H2 by green algae utilizes electrons originating from the photosynthetic oxidation of water and does not require metabolic intermediates. However, algal hydrogenases are extremely sensitive to O(2), which limits their usefulness in future commercial H2-production systems. We designed an experimental technique for the selection of O2-tolerant, H2-producing variants of Chlamydomonas reinhardtii based on the ability of wild-type cells to survive a short (20 min) exposure to metronidazole in the presence of controlled concentrations of O2. The number of survivors depends on the metronidazole concentration, light intensity, preinduction of the hydrogenase, and the presence or absence of O2. Finally, we demonstrate that some of the selected survivors in fact exhibit H2-production capacity that is less sensitive to O2 than the original wild-type population. |
Mendeley readers
The data shown below were compiled from readership statistics for 48 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 2% |
| Unknown | 47 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 25% |
| Researcher | 7 | 15% |
| Student > Bachelor | 5 | 10% |
| Student > Master | 5 | 10% |
| Student > Doctoral Student | 4 | 8% |
| Other | 6 | 13% |
| Unknown | 9 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 14 | 29% |
| Biochemistry, Genetics and Molecular Biology | 10 | 21% |
| Engineering | 6 | 13% |
| Chemistry | 4 | 8% |
| Immunology and Microbiology | 2 | 4% |
| Other | 4 | 8% |
| Unknown | 8 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 May 2021.
All research outputs
#3,271,154
of 22,782,096 outputs
Outputs from Applied Biochemistry and Biotechnology
#80
of 2,502 outputs
Outputs of similar age
#1,958
of 29,996 outputs
Outputs of similar age from Applied Biochemistry and Biotechnology
#2
of 16 outputs
Altmetric has tracked 22,782,096 research outputs across all sources so far. Compared to these this one has done well and is in the 84th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,502 research outputs from this source. They receive a mean Attention Score of 3.2. This one has done particularly well, scoring higher than 93% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 29,996 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 88% of its contemporaries.
We're also able to compare this research output to 16 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 81% of its contemporaries.