| Title |
Genetic Diversity Within and Among Feral Populations and Domesticated Strains of the Guppy (Poecilia reticulata) in Singapore
|
|---|---|
| Published in |
Marine Biotechnology, September 2002
|
| DOI | 10.1007/s10126-002-0007-z |
| Pubmed ID | |
| Authors |
Gideon Khoo, Kok Fang Lim, Damien K.Y. Gan, Fan Chen, Woon-Khiong Chan, Tit Meng Lim, Violet P.E. Phang |
| Abstract |
Genetic variability within and among feral populations and cultured strains of the guppy (Poecilia reticulata) was investigated by random amplification of polymorphic DNA (RAPD) fingerprinting. Feral guppies were collected from 6 isolated populations (BT, Bukit Timah; NS, Nee Soon; TS, Tuas; MF, Mount Faber; KR, Kranji; LI, laboratory-inbred feral line), while the Tuxedo and Green Variegated strains were sampled from 2 guppy farms in Singapore. Pairwise genetic distances analyzed by unweighted pair-group method with arithmetic means revealed distinct clustering of guppy individuals into their respective populations and strains. Percentage polymorphic loci ranged from 54.96% (TS) to 68.70% (KR), while average heterozygosity ranged from 0.220 (GV) to 0.271 (KR). In contrast, TS guppies had the highest (0.850) intrapopulation genetic similarity (S), whereas KR had the lowest (0.781). Among populations and strains, S ranged from 0.703 (between GV and LI) to 0.809 (between NS and MB). The GV strain S was closer to TX (0.784) than to the feral guppies. Bootstrapped genetic distance trees depicted 3 major nodes comprising BT-TS, NS-MF, and TX-GV. Principal coordinate analysis also differentiated the 6 feral populations from the 2 cultured strains. |
Mendeley readers
The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 4% |
| Mexico | 1 | 4% |
| Unknown | 25 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 22% |
| Student > Master | 5 | 19% |
| Student > Bachelor | 4 | 15% |
| Researcher | 2 | 7% |
| Student > Postgraduate | 2 | 7% |
| Other | 5 | 19% |
| Unknown | 3 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 16 | 59% |
| Biochemistry, Genetics and Molecular Biology | 4 | 15% |
| Unspecified | 1 | 4% |
| Veterinary Science and Veterinary Medicine | 1 | 4% |
| Environmental Science | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 4 | 15% |