Title |
The effect of INDEHISCENT point mutations on silique shatter resistance in oilseed rape (Brassica napus)
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Published in |
Theoretical and Applied Genetics, January 2018
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DOI | 10.1007/s00122-018-3051-4 |
Pubmed ID | |
Authors |
Janina Braatz, Hans-Joachim Harloff, Nazgol Emrani, Chirlon Elisha, Lars Heepe, Stanislav N. Gorb, Christian Jung |
Abstract |
This study elucidates the influence of indehiscent mutations on rapeseed silique shatter resistance. A phenotype with enlarged replum-valve joint area and altered cell dimensions in the dehiscence zone is described. Silique shattering is a major factor reducing the yield stability of oilseed rape (Brassica napus). Attempts to improve shatter resistance often include the use of mutations in target genes identified from Arabidopsis (Arabidopsis thaliana). A variety of phenotyping methods assessing the level of shatter resistance were previously described. However, a comparative and comprehensive evaluation of the methods has not yet been undertaken. We verified the increase of shatter resistance in indehiscent double knock-down mutants obtained by TILLING with a systematic approach comparing three independent phenotyping methods. A positive correlation of silique length and shatter resistance was observed and accounted for in the analyses. Microscopic studies ruled out the influence of different lignification patterns. Instead, we propose a model to explain increased shattering resistance of indehiscent rapeseed mutants by altered cell shapes and sizes within the contact surfaces of replum and valves. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 27 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 5 | 19% |
Researcher | 4 | 15% |
Student > Master | 2 | 7% |
Student > Bachelor | 2 | 7% |
Unspecified | 1 | 4% |
Other | 2 | 7% |
Unknown | 11 | 41% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 13 | 48% |
Biochemistry, Genetics and Molecular Biology | 2 | 7% |
Unspecified | 1 | 4% |
Unknown | 11 | 41% |