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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Guideline to reference gene selection for quantitative real-time PCR
|
|---|---|
| Published in |
Biochemical & Biophysical Research Communications, January 2004
|
| DOI | 10.1016/j.bbrc.2003.11.177 |
| Pubmed ID | |
| Authors |
Aleksandar Radonić, Stefanie Thulke, Ian M Mackay, Olfert Landt, Wolfgang Siegert, Andreas Nitsche |
| Abstract |
Today, quantitative real-time PCR is the method of choice for rapid and reliable quantification of mRNA transcription. However, for an exact comparison of mRNA transcription in different samples or tissues it is crucial to choose the appropriate reference gene. Recently glyceraldehyde 3-phosphate dehydrogenase and beta-actin have been used for that purpose. However, it has been reported that these genes as well as alternatives, like rRNA genes, are unsuitable references, because their transcription is significantly regulated in various experimental settings and variable in different tissues. Therefore, quantitative real-time PCR was used to determine the mRNA transcription profiles of 13 putative reference genes, comparing their transcription in 16 different tissues and in CCRF-HSB-2 cells stimulated with 12-O-tetradecanoylphorbol-13-acetate and ionomycin. Our results show that "Classical" reference genes are indeed unsuitable, whereas the RNA polymerase II gene was the gene with the most constant expression in different tissues and following stimulation in CCRF-HSB-2 cells. |
X Demographics
The data shown below were collected from the profiles of 15 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Denmark | 1 | 7% |
| Spain | 1 | 7% |
| Netherlands | 1 | 7% |
| Hungary | 1 | 7% |
| Austria | 1 | 7% |
| Unknown | 10 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 14 | 93% |
| Science communicators (journalists, bloggers, editors) | 1 | 7% |
Mendeley readers
The data shown below were compiled from readership statistics for 1,638 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 18 | 1% |
| United States | 13 | <1% |
| United Kingdom | 10 | <1% |
| Mexico | 9 | <1% |
| India | 9 | <1% |
| Spain | 8 | <1% |
| Brazil | 8 | <1% |
| Canada | 7 | <1% |
| France | 5 | <1% |
| Other | 46 | 3% |
| Unknown | 1505 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 415 | 25% |
| Researcher | 330 | 20% |
| Student > Master | 240 | 15% |
| Student > Bachelor | 137 | 8% |
| Student > Doctoral Student | 104 | 6% |
| Other | 250 | 15% |
| Unknown | 162 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 850 | 52% |
| Biochemistry, Genetics and Molecular Biology | 276 | 17% |
| Medicine and Dentistry | 125 | 8% |
| Environmental Science | 35 | 2% |
| Immunology and Microbiology | 30 | 2% |
| Other | 116 | 7% |
| Unknown | 206 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 20. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 December 2023.
All research outputs
#1,833,515
of 25,377,790 outputs
Outputs from Biochemical & Biophysical Research Communications
#228
of 26,637 outputs
Outputs of similar age
#3,723
of 143,820 outputs
Outputs of similar age from Biochemical & Biophysical Research Communications
#5
of 222 outputs
Altmetric has tracked 25,377,790 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 92nd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 26,637 research outputs from this source. They receive a mean Attention Score of 4.5. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 143,820 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 97% of its contemporaries.
We're also able to compare this research output to 222 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 98% of its contemporaries.