| Title |
Constitutive arginine-dependent nitric oxide synthase activity in different organs of pea seedlings during plant development
|
|---|---|
| Published in |
Planta, January 2006
|
| DOI | 10.1007/s00425-005-0205-9 |
| Pubmed ID | |
| Authors |
Francisco J. Corpas, Juan B. Barroso, Alfonso Carreras, Raquel Valderrama, José M. Palma, Ana M. León, Luisa M. Sandalio, Luis A del Río |
| Abstract |
Nitric oxide (NO) is an important signalling molecule in different animal and plant physiological processes. Little is known about its biological function in plants and on the enzymatic source or site of NO production during plant development. The endogenous NO production from L-arginine (NO synthase activity) was analyzed in leaves, stems and roots during plant development, using pea seedlings as a model. NOS activity was analyzed using a novel chemiluminescence-based assay which is more sensitive and specific than previous methods used in plant tissues. In parallel, NO accumulation was analyzed by confocal laser scanning microscopy using as fluorescent probes either DAF-2 DA or DAF-FM DA. A strong increase in NOS activity was detected in stems after 11 days growth, coinciding with the maximum stem elongation. The arginine-dependent NOS activity was constitutive and sensitive to aminoguanidine, a well-known irreversible inhibitor of animal NOS, and this NOS activity was differentially modulated depending on the plant organ and seedling developmental stage. In all tissues studied, NO was localized mainly in the vascular tissue (xylem) and epidermal cells and in root hairs. These loci of NO generation and accumulation suggest novel functions for NO in these cell types. |
Mendeley readers
The data shown below were compiled from readership statistics for 98 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Cyprus | 1 | 1% |
| Brazil | 1 | 1% |
| Belgium | 1 | 1% |
| China | 1 | 1% |
| Spain | 1 | 1% |
| United States | 1 | 1% |
| Unknown | 92 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 24 | 24% |
| Researcher | 18 | 18% |
| Student > Master | 10 | 10% |
| Student > Doctoral Student | 9 | 9% |
| Student > Bachelor | 5 | 5% |
| Other | 16 | 16% |
| Unknown | 16 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 59 | 60% |
| Biochemistry, Genetics and Molecular Biology | 11 | 11% |
| Engineering | 2 | 2% |
| Chemistry | 2 | 2% |
| Earth and Planetary Sciences | 1 | 1% |
| Other | 2 | 2% |
| Unknown | 21 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 April 2018.
All research outputs
#7,451,942
of 22,782,096 outputs
Outputs from Planta
#599
of 2,718 outputs
Outputs of similar age
#40,053
of 154,355 outputs
Outputs of similar age from Planta
#6
of 20 outputs
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So far Altmetric has tracked 2,718 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
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We're also able to compare this research output to 20 others from the same source and published within six weeks on either side of this one. This one is in the 20th percentile – i.e., 20% of its contemporaries scored the same or lower than it.