| Title |
Genomic-driven discovery of an amidinohydrolase involved in the biosynthesis of mediomycin A
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, January 2018
|
| DOI | 10.1007/s00253-017-8729-z |
| Pubmed ID | |
| Authors |
Fengxian Sun, Shumei Xu, Fang Jiang, Weiying Liu |
| Abstract |
Clethramycin (1) and mediomycin A (2) belong to the linear polyene polyketide (LPP) family of antibiotics that exhibit potent antifungal activity. Structural similarities exist between 1 and 2, except that 2 contains an amino moiety substituted for the guanidino moiety. Herein, the draft genome sequence of Streptomyces mediocidicus ATCC23936, a strain which produces both 1 and 2, was obtained through de novo sequencing. Bioinformatic analysis of the genome revealed a clethramycin (cle) gene cluster that contained 25 open reading frames (orfs). However, amidinohydrolase for 2 formation was not found in the cle gene cluster. Further genomic analysis revealed an amidinohydrolase MedX, which can hydrolyse the guanidino form (1) into the amino form (2) via heterologous co-expression of the cle cluster in Streptomyces lividans or by in vitro catalysis. These results also suggest the feasibility of engineering novel LPPs for drug discovery by manipulating the biosynthetic machinery of S. mediocidicus. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 67% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Science communicators (journalists, bloggers, editors) | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 27 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 30% |
| Researcher | 6 | 22% |
| Student > Bachelor | 3 | 11% |
| Other | 2 | 7% |
| Student > Doctoral Student | 2 | 7% |
| Other | 2 | 7% |
| Unknown | 4 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 26% |
| Biochemistry, Genetics and Molecular Biology | 5 | 19% |
| Chemistry | 3 | 11% |
| Medicine and Dentistry | 2 | 7% |
| Immunology and Microbiology | 1 | 4% |
| Other | 2 | 7% |
| Unknown | 7 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 January 2018.
All research outputs
#15,827,358
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#5,634
of 8,034 outputs
Outputs of similar age
#264,103
of 448,783 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#76
of 125 outputs
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