| Title |
Telomerase activity in HeLa cervical carcinoma cell line proliferation
|
|---|---|
| Published in |
Biogerontology, September 2006
|
| DOI | 10.1007/s10522-006-9043-9 |
| Pubmed ID | |
| Authors |
Milena Ivanković, Andrea Ćukušić, Ivana Gotić, Nikolina Škrobot, Mario Matijašić, Denis Polančec, Ivica Rubelj |
| Abstract |
Normal human somatic cells in culture have a limited dividing potential. This is due to DNA end replication problem, whereby telomeres shorten with each subsequent cell division. When a critical telomere length is reached cells enter senescence. To overcome this problem, immortal HeLa cell line express telomerase, an enzyme that prevents telomere shortening. Although immortal, the existence of non-dividing cells that do not incorporate (3)H-thymidine over 24 h of growth has been well documented in this cell line. Using DiI labeling and high-speed cell sorting, we have separated and analyzed fractions of HeLa cells that divided vigorously as well as those that cease divisions over several days in culture. We also analyzed telomerase activity in separated fractions and surprisingly, found that the fraction of cells that divided 0-1 time over 6 days in culture have several times higher endogenous telomerase activity than the fastest dividing fraction. Additionally, the non-growing fraction regains an overall high labeling index and low SA-beta-Gal activity when subcultured again. This phenomenon should be considered if telomerase inhibition is to be used as an approach to cancer therapy. In this paper we also discuss possible molecular mechanisms that underlie the observed results. |
Mendeley readers
The data shown below were compiled from readership statistics for 93 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Malaysia | 1 | 1% |
| Unknown | 92 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 32 | 34% |
| Student > Master | 8 | 9% |
| Student > Postgraduate | 7 | 8% |
| Researcher | 6 | 6% |
| Student > Ph. D. Student | 6 | 6% |
| Other | 11 | 12% |
| Unknown | 23 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 25 | 27% |
| Biochemistry, Genetics and Molecular Biology | 21 | 23% |
| Chemistry | 8 | 9% |
| Medicine and Dentistry | 4 | 4% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 3% |
| Other | 9 | 10% |
| Unknown | 23 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 October 2023.
All research outputs
#3,162,968
of 23,548,905 outputs
Outputs from Biogerontology
#128
of 671 outputs
Outputs of similar age
#6,644
of 68,017 outputs
Outputs of similar age from Biogerontology
#2
of 14 outputs
Altmetric has tracked 23,548,905 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 671 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.5. This one has done well, scoring higher than 80% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 68,017 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 14 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 85% of its contemporaries.