| Title |
Toxoplasma gondii antigen SAG2A differentially modulates IL-1β expression in resistant and susceptible murine peritoneal cells
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, January 2018
|
| DOI | 10.1007/s00253-018-8759-1 |
| Pubmed ID | |
| Authors |
Jamilly Azevedo Leal-Sena, Jane Lima dos Santos, Thaise Anne Rocha dos Santos, Edson Mário de Andrade, Tiago Antônio de Oliveira Mendes, Juliano Oliveira Santana, Tiago Wilson Patriarca Mineo, José Roberto Mineo, Jair Pereira da Cunha-Júnior, Carlos Priminho Pirovani |
| Abstract |
The cell surface of Toxoplasma gondii is covered by antigens (SAGs) from the SRS family anchored by glycosylphosphatidylinositol (GPI) and includes antigens from the SAG2 family. Among these, the SAG2A surface antigen shows great potential in activating humoral responses and has been used in characterizing the acute phase of infection and in the serological diagnosis of toxoplasmosis. In this study, we aimed to evaluate rSAG2A-induced proteins in BALB/c and C57BL/c mice macrophages and to evaluate the phenotypic polarization induced in the process. We treated the peritoneal macrophages from mouse strains that were resistant or susceptible to T. gondii with rSAG2A to analyze their proteomic profile by mass spectrometry and systems biology. We also examined the gene expression of these cells by RT-qPCR using the phenotypic markers of M1 and M2 macrophages. Differences were observed in the expression of proteins involved in the inflammatory process in both resistant and susceptible cells, and macrophages were preferentially induced to obtain a pro-inflammatory immune response (M1) via the overexpression of IL-1β in mice susceptible to this parasite. These data suggest that the SAG2A antigen induces phenotypic and classical activation of macrophages in both resistant and susceptible strains of mice during the acute phase of the disease. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 37 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 10 | 27% |
| Student > Ph. D. Student | 6 | 16% |
| Professor | 5 | 14% |
| Student > Doctoral Student | 2 | 5% |
| Student > Bachelor | 1 | 3% |
| Other | 3 | 8% |
| Unknown | 10 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 8 | 22% |
| Agricultural and Biological Sciences | 5 | 14% |
| Veterinary Science and Veterinary Medicine | 4 | 11% |
| Biochemistry, Genetics and Molecular Biology | 3 | 8% |
| Computer Science | 2 | 5% |
| Other | 2 | 5% |
| Unknown | 13 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 January 2018.
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#21,608,038
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#6,994
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#385,632
of 448,622 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#111
of 125 outputs
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