| Title |
Metallothionein-3 (MT-3) in the Human Adrenal Cortex and its Disorders
|
|---|---|
| Published in |
Endocrine Pathology, November 2013
|
| DOI | 10.1007/s12022-013-9280-9 |
| Pubmed ID | |
| Authors |
Saulo J. A. Felizola, Yasuhiro Nakamura, Yuki Arata, Kazue Ise, Fumitoshi Satoh, William E. Rainey, Sanae Midorikawa, Shinichi Suzuki, Hironobu Sasano |
| Abstract |
Metallothionein-3 (MT-3) is an intracellular, low molecular weight protein mainly distributed in the central nervous system but also in various peripheral organs and several types of human neoplasms. However, details of MT-3 expression have not been examined in human adrenal cortex and its disorders. The mRNA levels of MT-3 were first evaluated by quantitative RT-PCR (qPCR) in adrenocortical aldosterone-producing adenoma (APA: 11) and cortisol-producing adenoma (CPA: 14). In addition, MT-3 immunohistochemistry was performed in non-pathological adrenal glands (NA: 19), idiopathic hyperaldosteronism (IHA: 10), APA (20), CPA (24), adjacent non-neoplastic adrenal glands of adenoma (AAG: 20), and adrenocortical carcinoma (ACC: 8). H295R cells were also treated with angiotensin-II or forskolin in a time-dependent manner, and the changes of MT-3 mRNA levels were evaluated by qPCR. Results of qPCR analysis demonstrated that MT-3 mRNA levels were significantly higher in APA than CPA (P = 0.0004). MT-3 immunoreactivity was detected in the zona glomerulosa of NA, IHA, and AAG, as well as in APA, CPA, and ACC. When treated with angiotensin-II and forskolin, MT-3 mRNA levels reached a peak by 12 h in H295R cells, with significantly higher levels compared to control non-treated cells (P < 0.01). The presence of MT-3 in the ZG of NA, IHA, and AAG, as well as APA may imply a role in the pathophysiology of aldosterone-producing tissues. |
Mendeley readers
The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 23 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 6 | 26% |
| Student > Ph. D. Student | 3 | 13% |
| Student > Bachelor | 2 | 9% |
| Other | 2 | 9% |
| Student > Doctoral Student | 1 | 4% |
| Other | 3 | 13% |
| Unknown | 6 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 5 | 22% |
| Biochemistry, Genetics and Molecular Biology | 3 | 13% |
| Agricultural and Biological Sciences | 3 | 13% |
| Chemistry | 2 | 9% |
| Immunology and Microbiology | 1 | 4% |
| Other | 2 | 9% |
| Unknown | 7 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 September 2023.
All research outputs
#7,689,410
of 23,394,907 outputs
Outputs from Endocrine Pathology
#103
of 355 outputs
Outputs of similar age
#92,331
of 305,428 outputs
Outputs of similar age from Endocrine Pathology
#2
of 5 outputs
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So far Altmetric has tracked 355 research outputs from this source. They receive a mean Attention Score of 4.0. This one has gotten more attention than average, scoring higher than 58% of its peers.
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