| Title |
Polymerase chain reaction-based identification of Plasmodium (Huffia) elongatum, with remarks on species identity of haemosporidian lineages deposited in GenBank
|
|---|---|
| Published in |
Parasitology Research, February 2008
|
| DOI | 10.1007/s00436-008-0892-9 |
| Pubmed ID | |
| Authors |
Gediminas Valkiūnas, Pavel Zehtindjiev, Dimitar Dimitrov, Asta Križanauskienė, Tatjana A. Iezhova, Staffan Bensch |
| Abstract |
Numerous lineages of avian malaria parasites of the genus Plasmodium have been deposited in GenBank. However, only 11 morphospecies of Plasmodium have been linked to these lineages. Such linking is important because it provides opportunities to combine the existing knowledge of traditional parasitology with novel genetic information of these parasites obtained by molecular techniques. This study linked one mitochondrial cytochrome b (cyt b) gene lineage with morphospecies Plasmodium (Huffia) elongatum, a cosmopolitan avian malaria parasite which causes lethal disease in some birds. One species of Plasmodium (mitochondrial cyt b gene lineage P-GRW6) was isolated from naturally infected adult great reed warblers (Acrocephalus arundinaceus) and inoculated to one naive juvenile individual of the same host species. Heavy parasitaemia developed in the subinoculated bird, which enabled identification of the morphospecies and deposition of its voucher specimens. The parasite of this lineage belongs to P. elongatum. Illustrations of blood stages of this parasite are given. Other lineages closely related to P. elongatum were identified. The validity of the subgenus Huffia is supported by phylogenetic analysis. Mitochondrial cyt b gene lineages, with GenBank accession nos. AF069611 and AY733088, belong to Plasmodium cathemerium and P. elongatum, respectively; these lineages have been formerly attributed to P. elongatum and P. relictum, respectively. Some other incorrect species identifications of avian haematozoa in GenBank have been identified. We propose a strategy to minimise the number of such mistakes in GenBank in the future. |
Mendeley readers
The data shown below were compiled from readership statistics for 94 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 2% |
| Colombia | 1 | 1% |
| Malaysia | 1 | 1% |
| Germany | 1 | 1% |
| Lithuania | 1 | 1% |
| Brazil | 1 | 1% |
| Unknown | 87 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 20 | 21% |
| Student > Ph. D. Student | 16 | 17% |
| Student > Master | 15 | 16% |
| Student > Bachelor | 7 | 7% |
| Student > Doctoral Student | 4 | 4% |
| Other | 16 | 17% |
| Unknown | 16 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 52 | 55% |
| Environmental Science | 7 | 7% |
| Biochemistry, Genetics and Molecular Biology | 5 | 5% |
| Veterinary Science and Veterinary Medicine | 4 | 4% |
| Immunology and Microbiology | 2 | 2% |
| Other | 5 | 5% |
| Unknown | 19 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 January 2017.
All research outputs
#7,451,942
of 22,782,096 outputs
Outputs from Parasitology Research
#621
of 3,782 outputs
Outputs of similar age
#43,319
of 158,126 outputs
Outputs of similar age from Parasitology Research
#4
of 25 outputs
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