| Title |
Functional Characterization of Novel Circular RNA Molecule, circzip-2 and Its Synthesizing Gene zip-2 in C. elegans Model of Parkinson’s Disease
|
|---|---|
| Published in |
Molecular Neurobiology, January 2018
|
| DOI | 10.1007/s12035-018-0903-5 |
| Pubmed ID | |
| Authors |
Lalit Kumar, Shamsuzzama, Pooja Jadiya, Rizwanul Haque, Shikha Shukla, Aamir Nazir |
| Abstract |
Circular RNAs (circRNAs) are peculiar non-coding RNA molecules which are known to be present across taxa. Considering the body of evidence that establishes critical functions of non-coding RNA molecules, we endeavored to study circRNAs in the context of Parkinson's disease (PD). Employing transgenic C. elegans model of PD, we used RNase R-mediated cleavage of linear RNA followed by divergent primer-based amplifications towards identifying circzip-2, a novel circRNA molecule. We went on to sequence circzip-2 which is synthesized from functionally important gene zip-2. Studying RNAi-induced knockdown conditions of zip-2, we observed a reduced aggregation of α-synuclein protein along with an enhanced lifespan of the worms. We further carried out transcriptome analysis of zip-2 silenced worms, which suggested that zip-2 might be functioning via Daf-16 pathway. Further interaction studies revealed that circzip-2 possibly sponges microRNA molecule miR-60 towards asserting an important role in various processes associated with PD. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 25% |
| Unknown | 3 | 75% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Scientists | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 43 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 13 | 30% |
| Student > Bachelor | 7 | 16% |
| Student > Master | 5 | 12% |
| Researcher | 5 | 12% |
| Student > Postgraduate | 2 | 5% |
| Other | 1 | 2% |
| Unknown | 10 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 14 | 33% |
| Neuroscience | 6 | 14% |
| Nursing and Health Professions | 3 | 7% |
| Medicine and Dentistry | 3 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 5% |
| Other | 5 | 12% |
| Unknown | 10 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 February 2019.
All research outputs
#14,965,143
of 23,018,998 outputs
Outputs from Molecular Neurobiology
#1,952
of 3,487 outputs
Outputs of similar age
#256,006
of 441,019 outputs
Outputs of similar age from Molecular Neurobiology
#51
of 108 outputs
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