| Title |
Ultrasonication-assisted spray ionization mass spectrometry for the analysis of biomolecules in solution
|
|---|---|
| Published in |
Journal of the American Society for Mass Spectrometry, November 2011
|
| DOI | 10.1016/j.jasms.2010.04.021 |
| Pubmed ID | |
| Authors |
Tsung-Yi Chen, Jia-Yi Lin, Jen-Yi Chen, Yu-Chie Chen |
| Abstract |
In this paper, we describe a novel technique--ultrasonication-assisted spray ionization (UASI)--for the generation of singly charged and multiply charged gas-phase ions of biomolecules (e.g., amino acids, peptides, and proteins) from solution; this method employs a low-frequency ultrasonicator (ca. 40 kHz) in place of the high electric field required for electrospray ionization. When a capillary inlet is immersed into a sample solution within a vial subjected to ultrasonication, the solution is continually directed to the capillary outlet as a result of ultrasonication-assisted capillary action; an ultrasonic spray of the sample solution is emitted at the outlet of the tapered capillary, leading to the ready generation of gas-phase ions. Using an ion trap mass spectrometer, we found that singly charged amino acid and multiply charged peptides/proteins ions were generated through this single-step operation, which is both straightforward and extremely simple to perform. The setup is uncomplicated: only a low-frequency ultrasonicator and a tapered capillary are required to perform UASI. The mass spectra of the multiply charged peptides and proteins obtained from sample solutions subjected to UASI resemble those observed in ESI mass spectra. |
Mendeley readers
The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 3% |
| Taiwan | 1 | 3% |
| Unknown | 31 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 24% |
| Researcher | 8 | 24% |
| Student > Bachelor | 4 | 12% |
| Student > Doctoral Student | 3 | 9% |
| Other | 2 | 6% |
| Other | 3 | 9% |
| Unknown | 5 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 16 | 48% |
| Agricultural and Biological Sciences | 4 | 12% |
| Engineering | 3 | 9% |
| Computer Science | 1 | 3% |
| Arts and Humanities | 1 | 3% |
| Other | 2 | 6% |
| Unknown | 6 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 March 2023.
All research outputs
#8,535,684
of 25,374,917 outputs
Outputs from Journal of the American Society for Mass Spectrometry
#1,227
of 3,835 outputs
Outputs of similar age
#71,829
of 245,491 outputs
Outputs of similar age from Journal of the American Society for Mass Spectrometry
#65
of 172 outputs
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