| Title |
Cloning and characterization of a specific UDP-glycosyltransferase gene induced by DON and Fusarium graminearum
|
|---|---|
| Published in |
Plant Cell Reports, January 2018
|
| DOI | 10.1007/s00299-018-2257-x |
| Pubmed ID | |
| Authors |
Lanfei Zhao, Xin Ma, Peisen Su, Wenyang Ge, Hongyan Wu, Xiuxiu Guo, Anfei Li, Hongwei Wang, Lingrang Kong |
| Abstract |
TaUGT5: can reduce the proliferation and destruction of F. graminearum and enhance the ability of FHB resistance in wheat. Deoxynivalenol (DON) is one of the most important toxins produced by Fusarium species that enhances the spread of the pathogen in the host. As a defense, the UDP-glycosyltransferase (UGT) family has been deduced to transform DON into the less toxic form DON-3-O-glucoside (D3G), but the specific gene member in wheat that is responsible for Fusarium head blight (FHB) resistance has been little investigated and proved. In this study, a DON and Fusarium graminearum responsive gene TaUGT5, which is specific for resistant cultivars, was cloned with a 1431 bp open reading frame (ORF) encoding 476 amino acids in Sumai3. TaUGT5 is located on chromosome 2B, which has been confirmed in nulli-tetrasomic lines of Chinese Spring (CS) and is solely expressed among three homologs on the A, B and D genomes. Over-expression of this gene in Arabidopsis conferred enhanced tolerance when grown on agar plates that contain DON. Similarly, the coleoptiles of wheat over-expressing TaUGT5 showed more resistance to F. graminearum, evidencing reduced proliferation and destruction of plant tissue by the pathogen. However, the disease resistance in spikes was not as significant as that on coleoptile compared with wild-type plants. A subcellular localization analysis revealed that TaUGT5 was localized on the plasma membrane of tobacco leaf epidermal cells. It is possible that TaUGT5 could enhance tolerance to DON, protect the plant cell from the pathogen infection and result in better maintenance of the cell structure, which slows down pathogen proliferation in plant tissue. |
X Demographics
The data shown below were collected from the profiles of 17 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 2 | 12% |
| Canada | 1 | 6% |
| Brazil | 1 | 6% |
| Ecuador | 1 | 6% |
| Mexico | 1 | 6% |
| United States | 1 | 6% |
| Spain | 1 | 6% |
| Italy | 1 | 6% |
| Unknown | 8 | 47% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 12 | 71% |
| Scientists | 5 | 29% |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 37 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 22% |
| Researcher | 7 | 19% |
| Student > Master | 3 | 8% |
| Student > Bachelor | 3 | 8% |
| Other | 2 | 5% |
| Other | 5 | 14% |
| Unknown | 9 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 15 | 41% |
| Biochemistry, Genetics and Molecular Biology | 10 | 27% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
| Unknown | 11 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 May 2020.
All research outputs
#4,020,404
of 24,208,207 outputs
Outputs from Plant Cell Reports
#235
of 2,287 outputs
Outputs of similar age
#85,852
of 449,012 outputs
Outputs of similar age from Plant Cell Reports
#10
of 32 outputs
Altmetric has tracked 24,208,207 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,287 research outputs from this source. They receive a mean Attention Score of 4.1. This one has done well, scoring higher than 89% of its peers.
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We're also able to compare this research output to 32 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.