| Title |
Time-course responses of ileal and fecal microbiota and metabolite profiles to antibiotics in cannulated pigs
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, January 2018
|
| DOI | 10.1007/s00253-018-8774-2 |
| Pubmed ID | |
| Authors |
Kan Gao, Yu Pi, Yu Peng, Chun-Long Mu, Wei-Yun Zhu |
| Abstract |
We investigated the time-course effects of therapeutic antibiotics on intestinal microbial composition and metabolism in an ileal-cannulated pig model. Sixteen ileal-cannulated piglets (12 ± 0.5 kg) were assigned to two groups (n = 8) and fed standard diets with or without antibiotics. At 4 days before, and at days 2, 7, and 13 after antibiotic administration, ileal and fecal samples were collected for analysis of microbiota composition via 16S rRNA MiSeq sequencing and metabolites (short-chain fatty acids, biogenic amines, and indole). It was found that Lactobacillus and Bifidobacterium had decreased by an average 2.68-fold and 508-fold in ileum on days 2-13, and by an average 45.08-fold and 71.50-fold in feces on days 7-13 (P < 0.05). Escherichia/Shigella had increased by an average 265-fold in ileum on days 2-13, and by an average 36.70-fold in feces on days 7-13 (P < 0.05). Acetate concentration had decreased in ileum by an average 2.88-fold on days 2-13, and by 1.83-fold in feces on day 7 (P < 0.05). Cadaverine concentration had increased by an average 7.03-fold in ileum on days 2-13, and by an average 9.96-fold in feces on days 7-13 (P < 0.05), and fecal indole concentration had increased by an average 2.51-fold on days 7-13 (P < 0.05). Correlation analysis between significant microbes and metabolites indicated that the antibiotic-induced microbiota shift appeared to result in the changes of intestinal metabolism. In conclusion, antibiotic administration led to dynamic changes in microbial communities and metabolism in ileum and feces, with ileal microbiota being more prone to shift than fecal microbiota. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 63 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 63 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 14 | 22% |
| Student > Ph. D. Student | 8 | 13% |
| Student > Master | 8 | 13% |
| Student > Bachelor | 3 | 5% |
| Other | 3 | 5% |
| Other | 8 | 13% |
| Unknown | 19 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 18 | 29% |
| Veterinary Science and Veterinary Medicine | 5 | 8% |
| Biochemistry, Genetics and Molecular Biology | 5 | 8% |
| Immunology and Microbiology | 4 | 6% |
| Medicine and Dentistry | 3 | 5% |
| Other | 7 | 11% |
| Unknown | 21 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 15. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 March 2023.
All research outputs
#2,446,751
of 25,401,381 outputs
Outputs from Applied Microbiology and Biotechnology
#228
of 8,301 outputs
Outputs of similar age
#54,888
of 450,290 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#8
of 134 outputs
Altmetric has tracked 25,401,381 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,301 research outputs from this source. They receive a mean Attention Score of 4.4. This one has done particularly well, scoring higher than 97% of its peers.
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