| Title |
Enzymatic synthesis of avermectin B1a glycosides for the effective prevention of the pine wood nematode Bursaphelenchus xylophilus
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, January 2018
|
| DOI | 10.1007/s00253-018-8764-4 |
| Pubmed ID | |
| Authors |
Ha-Young Choi, Nguyen Van Minh, Jae Min Choi, Jae Yoon Hwang, Sang-Tae Seo, Seung-Kyu Lee, Won-Gon Kim |
| Abstract |
Avermectin produced by Streptomyces avermitilis is an anti-nematodal agent against the pine wood nematode Bursaphelenchus xylophilus. However, its potential usage is limited by its poor water solubility. For this reason, continuous efforts are underway to produce new derivatives that are more water soluble. Here, the enzymatic glycosylation of avermectin was catalyzed by uridine diphosphate (UDP)-glycosyltransferase from Bacillus licheniformis with various UDP sugars. As a result, the following four avermectin B1a glycosides were produced: avermectin B1a 4″-β-D-glucoside, avermectin B1a 4″-β-D-galactoside, avermectin B1a 4″-β-L-fucoside, and avermectin B1a 4″-β-2-deoxy-D-glucoside. The avermectin B1a glycosides were structurally analyzed based on HR-ESI MS and 1D and 2D nuclear magnetic resonance spectra, and the anti-nematodal effect of avermectin B1a 4″-β-D-glucoside was found to exhibit the highest activity (IC50 = 0.23 μM), which was approximately 32 times greater than that of avermectin B1a (IC50 = 7.30 μM), followed by avermectin B1a 4″-β-2-deoxy-D-glucoside (IC50 = 0.69 μM), avermectin B1a 4″-β-L-fucoside (IC50 = 0.89 μM), and avermectin B1a 4″-β-D-galactoside (IC50 = 1.07 μM). These results show that glycosylation of avermectin B1a effectively enhances its in vitro anti-nematodal activity and that avermectin glycosides can be further applied for treating infestations of the pine wood nematode B. xylophilus. |
X Demographics
The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 3 | 60% |
| Unknown | 2 | 40% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 80% |
| Science communicators (journalists, bloggers, editors) | 1 | 20% |
Mendeley readers
The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 19 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 21% |
| Student > Ph. D. Student | 4 | 21% |
| Student > Master | 2 | 11% |
| Lecturer | 1 | 5% |
| Unspecified | 1 | 5% |
| Other | 2 | 11% |
| Unknown | 5 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 47% |
| Unspecified | 1 | 5% |
| Biochemistry, Genetics and Molecular Biology | 1 | 5% |
| Environmental Science | 1 | 5% |
| Business, Management and Accounting | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 5 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 August 2018.
All research outputs
#15,190,918
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#5,484
of 8,034 outputs
Outputs of similar age
#248,192
of 448,547 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#74
of 133 outputs
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