| Title |
A labile point in mutant amphotericin polyketide synthases
|
|---|---|
| Published in |
Biotechnology Techniques, January 2011
|
| DOI | 10.1007/s10529-011-0538-3 |
| Pubmed ID | |
| Authors |
Naseem Khan, Bernard Rawlings, Patrick Caffrey |
| Abstract |
Streptomyces nodosus produces the antifungal polyene amphotericin B. Numerous modifications of the amphotericin polyketide synthase have yielded new analogues. However, previous inactivation of the ketoreductase in module 10 resulted in biosynthesis of truncated polyketides. Here we show that modules downstream of this domain remain intact. Therefore, loss of ketoreductase-10 activity is sufficient to cause early chain termination. This modification creates a labile point in cycle 11 of the polyketide biosynthetic pathway. Non-extendable intermediates are released to accumulate as polyenyl-pyrones. |
Mendeley readers
The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 23 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 13 | 57% |
| Student > Master | 5 | 22% |
| Student > Bachelor | 2 | 9% |
| Student > Postgraduate | 1 | 4% |
| Unknown | 2 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 8 | 35% |
| Agricultural and Biological Sciences | 7 | 30% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 9% |
| Computer Science | 1 | 4% |
| Biochemistry, Genetics and Molecular Biology | 1 | 4% |
| Other | 2 | 9% |
| Unknown | 2 | 9% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 April 2013.
All research outputs
#5,548,758
of 25,728,855 outputs
Outputs from Biotechnology Techniques
#266
of 2,767 outputs
Outputs of similar age
#35,340
of 196,090 outputs
Outputs of similar age from Biotechnology Techniques
#8
of 30 outputs
Altmetric has tracked 25,728,855 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,767 research outputs from this source. They receive a mean Attention Score of 3.9. This one has gotten more attention than average, scoring higher than 73% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 196,090 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 77% of its contemporaries.
We're also able to compare this research output to 30 others from the same source and published within six weeks on either side of this one. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.