| Title |
The extracellular patch clamp: A method for resolving currents through individual open channels in biological membranes
|
|---|---|
| Published in |
Pflügers Archiv - European Journal of Physiology, July 1978
|
| DOI | 10.1007/bf00584247 |
| Pubmed ID | |
| Authors |
Erwin Neher, Bert Sakmann, Joe Henry Steinbach |
| Abstract |
The current contributions of individual ionic channels can be measured by electrically isolating a small patch of membrane. To do this, the tip of a small pipette is brought into close contact with an enzymatically cleaned membrane of a hypersensitive amphibian or mammalian muscle fiber. Current flowing through the pipette is measured. If the pipette contains cholinergic agonist at mu-molar concentrations, square pulse current waveforms can be observed which represent the activation of individual acetylcholine-receptor channels. The square pulses have amplitudes of 1 to 3 pA and durations of 10--100ms. In order to obtain the necessary resolution, a delicate compromise had to be found between different experimental parameters. Pipettes with 1--3 micrometer internal diameter and a steep final taper had to be used, extensive enzyme treatment was necessary, and conditions had be to found in which channels open at a relatively low frequency. |
Mendeley readers
The data shown below were compiled from readership statistics for 296 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 5 | 2% |
| United States | 5 | 2% |
| Germany | 3 | 1% |
| Italy | 2 | <1% |
| Sweden | 1 | <1% |
| Portugal | 1 | <1% |
| China | 1 | <1% |
| Brazil | 1 | <1% |
| Unknown | 277 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 85 | 29% |
| Researcher | 39 | 13% |
| Student > Master | 38 | 13% |
| Student > Bachelor | 33 | 11% |
| Student > Doctoral Student | 17 | 6% |
| Other | 47 | 16% |
| Unknown | 37 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 81 | 27% |
| Neuroscience | 34 | 11% |
| Engineering | 31 | 10% |
| Biochemistry, Genetics and Molecular Biology | 27 | 9% |
| Chemistry | 20 | 7% |
| Other | 53 | 18% |
| Unknown | 50 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 February 2024.
All research outputs
#2,940,048
of 26,017,215 outputs
Outputs from Pflügers Archiv - European Journal of Physiology
#91
of 2,119 outputs
Outputs of similar age
#196
of 5,466 outputs
Outputs of similar age from Pflügers Archiv - European Journal of Physiology
#1
of 8 outputs
Altmetric has tracked 26,017,215 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,119 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one has done particularly well, scoring higher than 94% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 5,466 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 94% of its contemporaries.
We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them