| Title |
Rapid conjugative mobilization of a 100 kb segment of Bacillus subtilis chromosomal DNA is mediated by a helper plasmid with no ability for self-transfer
|
|---|---|
| Published in |
Microbial Cell Factories, January 2018
|
| DOI | 10.1186/s12934-017-0855-x |
| Pubmed ID | |
| Authors |
Megumi Miyano, Kosei Tanaka, Shu Ishikawa, Shinji Takenaka, Andrés Miguel-Arribas, Wilfried J. J. Meijer, Ken-ichi Yoshida |
| Abstract |
The conjugative plasmid, pLS20, isolated from Bacillus subtilis natto, has an outstanding capacity for rapid self-transfer. In addition, it can function as a helper plasmid, mediating the mobilization of an independently replicating co-resident plasmid. In this study, the oriT sequence of pLS20cat (oriTLS20) was eliminated to obtain the plasmid, pLS20catΔoriT. This resulted in the complete loss of the conjugative transfer of the plasmid but still allowed it to mobilize a co-resident mobilizable plasmid. Moreover, pLS20catΔoriT was able to mobilize longer DNA segments, up to 113 kb of chromosomal DNA containing oriTLS20, after mixing the liquid cultures of the donor and recipient for only 15 min. The chromosomal DNA mobilization mediated by pLS20catΔoriT will allow us to develop a novel genetic tool for the rapid, easy, and repetitive mobilization of longer DNA segments into a recipient chromosome. |
X Demographics
The data shown below were collected from the profiles of 14 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 3 | 21% |
| Netherlands | 2 | 14% |
| United States | 2 | 14% |
| Sweden | 1 | 7% |
| Norway | 1 | 7% |
| Spain | 1 | 7% |
| Chile | 1 | 7% |
| Australia | 1 | 7% |
| Unknown | 2 | 14% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 10 | 71% |
| Members of the public | 4 | 29% |
Mendeley readers
The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 30 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 37% |
| Student > Master | 4 | 13% |
| Researcher | 3 | 10% |
| Student > Bachelor | 3 | 10% |
| Other | 1 | 3% |
| Other | 2 | 7% |
| Unknown | 6 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 16 | 53% |
| Agricultural and Biological Sciences | 4 | 13% |
| Engineering | 2 | 7% |
| Medicine and Dentistry | 1 | 3% |
| Unknown | 7 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 November 2021.
All research outputs
#4,018,216
of 24,593,959 outputs
Outputs from Microbial Cell Factories
#193
of 1,740 outputs
Outputs of similar age
#84,374
of 450,422 outputs
Outputs of similar age from Microbial Cell Factories
#6
of 36 outputs
Altmetric has tracked 24,593,959 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,740 research outputs from this source. They receive a mean Attention Score of 4.8. This one has done well, scoring higher than 88% of its peers.
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We're also able to compare this research output to 36 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 86% of its contemporaries.