| Title |
Increasing charge while preserving noncovalent protein complexes for ESI-MS
|
|---|---|
| Published in |
Journal of the American Society for Mass Spectrometry, November 2008
|
| DOI | 10.1016/j.jasms.2008.11.013 |
| Pubmed ID | |
| Authors |
Shirley H. Lomeli, Sheng Yin, Rachel R. Ogorzalek Loo, Joseph A. Loo |
| Abstract |
Increased multiple charging of native proteins and noncovalent protein complexes is observed in electrospray ionization (ESI) mass spectra obtained from nondenaturing protein solutions containing up to 1% (vol/vol) m-nitrobenzyl alcohol (m-NBA). The increases in charge ranged from 8% for the 690 kDa alpha(7)beta(7)beta(7)alpha(7) 20S proteasome complex to 48% additional charge for the zinc-bound 29 kDa carbonic anhydrase-II protein. No dissociation of the noncovalently bound ligands/subunits was observed upon the addition of m-NBA. It is not clear if the enhanced charging is related to altered surface tension as proposed in the "supercharging" model of Iavarone and Williams (Iavarone, A. T.; Williams, E. R. J. Am. Chem. Soc.2003, 125, 2319-2327). However, more highly charged noncovalent protein complexes have utility in relaxing slightly the mass-to-charge (m/z) requirements of the mass spectrometer for detection and will be effective for enhancing the efficiency for tandem mass spectrometry studies of protein complexes. |
Mendeley readers
The data shown below were compiled from readership statistics for 116 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 2% |
| United States | 2 | 2% |
| Germany | 1 | <1% |
| France | 1 | <1% |
| Unknown | 110 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 40 | 34% |
| Researcher | 22 | 19% |
| Student > Bachelor | 14 | 12% |
| Student > Master | 14 | 12% |
| Professor | 5 | 4% |
| Other | 11 | 9% |
| Unknown | 10 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 66 | 57% |
| Agricultural and Biological Sciences | 16 | 14% |
| Biochemistry, Genetics and Molecular Biology | 14 | 12% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 3% |
| Computer Science | 1 | <1% |
| Other | 2 | 2% |
| Unknown | 13 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 March 2021.
All research outputs
#8,534,528
of 25,373,627 outputs
Outputs from Journal of the American Society for Mass Spectrometry
#1,226
of 3,833 outputs
Outputs of similar age
#51,325
of 178,574 outputs
Outputs of similar age from Journal of the American Society for Mass Spectrometry
#8
of 20 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,833 research outputs from this source. They receive a mean Attention Score of 3.8. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
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