| Title |
Selection of the optimum electrospray voltage for gradient elution LC-MS measurements
|
|---|---|
| Published in |
Journal of the American Society for Mass Spectrometry, December 2008
|
| DOI | 10.1016/j.jasms.2008.12.004 |
| Pubmed ID | |
| Authors |
Ioan Marginean, Ryan T. Kelly, Ronald J. Moore, David C. Prior, Brian L. LaMarche, Keqi Tang, Richard D. Smith |
| Abstract |
Changes in liquid composition during gradient elution liquid chromatography (LC) coupled to mass spectrometry (MS) analyses affect the electrospray operation. To establish methodologies for judicious selection of the electrospray voltage, we monitored in real time the effect of the LC gradient on the spray current. The optimum range of the electrospray voltage decreased as the concentration of organic solvent in the eluent increased during reversed-phase LC analyses. These results and related observations provided the means to rationally select the voltage to ensure effective electrospray operation throughout gradient-elution LC separations. For analyses in which the electrospray was operated at constant voltage, a small run-to-run variation in the spray current was observed, indicating a changing electric field resulting from fouling or degradation of the emitter. Algorithms using feedback from spray current measurements that can maintain the electrospray voltage within the optimum operating range throughout gradient elution LC-MS were evaluated. The electrospray operation with voltage regulation and at a constant, judiciously selected voltage during gradient elution LC-MS measurements produced data with similar reproducibility. |
Mendeley readers
The data shown below were compiled from readership statistics for 55 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Italy | 1 | 2% |
| France | 1 | 2% |
| Unknown | 53 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 13 | 24% |
| Student > Ph. D. Student | 12 | 22% |
| Student > Master | 8 | 15% |
| Student > Bachelor | 4 | 7% |
| Student > Doctoral Student | 3 | 5% |
| Other | 7 | 13% |
| Unknown | 8 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 18 | 33% |
| Biochemistry, Genetics and Molecular Biology | 9 | 16% |
| Agricultural and Biological Sciences | 5 | 9% |
| Medicine and Dentistry | 3 | 5% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 5% |
| Other | 9 | 16% |
| Unknown | 8 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 February 2024.
All research outputs
#3,802,284
of 25,394,764 outputs
Outputs from Journal of the American Society for Mass Spectrometry
#254
of 3,836 outputs
Outputs of similar age
#17,882
of 179,038 outputs
Outputs of similar age from Journal of the American Society for Mass Spectrometry
#2
of 21 outputs
Altmetric has tracked 25,394,764 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,836 research outputs from this source. They receive a mean Attention Score of 3.8. This one has done particularly well, scoring higher than 90% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 179,038 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 21 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 85% of its contemporaries.