| Title |
A laccase from the medicinal mushroom Ganoderma lucidum
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, April 2006
|
| DOI | 10.1007/s00253-006-0314-9 |
| Pubmed ID | |
| Authors |
H. X. Wang, T. B. Ng |
| Abstract |
A protein demonstrating laccase activity and potent inhibitory activity towards human immunodeficiency virus (HIV)-1 reverse transcriptase (IC50 1.2 microM) was isolated from fresh fruiting bodies of the medicinal mushroom Ganoderma lucidum. The laccase had a novel N-terminal sequence and a molecular mass of 75 kDa, which is higher than the range (55-56 kDa) reported for most other mushroom laccases. It was isolated by sequential chromatography on DEAE-cellulose and Affi-gel blue gel and adsorption on Con A-Sepharose. Unlike some of the previously isolated laccases, it was adsorbed only on Con A-Sepharose. The enzyme required a pH of 3-5 and a temperature of 70 degrees C to exhibit maximal activity. Minimal activity was detected at pH 6 and 7. Activity was undetectable at pH 8 and 9 and after exposure to 100 degrees C for 10 min. |
Mendeley readers
The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 1% |
| Russia | 1 | 1% |
| Denmark | 1 | 1% |
| Unknown | 64 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 15% |
| Student > Bachelor | 10 | 15% |
| Researcher | 9 | 13% |
| Student > Master | 9 | 13% |
| Professor > Associate Professor | 5 | 7% |
| Other | 13 | 19% |
| Unknown | 11 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 32 | 48% |
| Biochemistry, Genetics and Molecular Biology | 6 | 9% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 4% |
| Medicine and Dentistry | 2 | 3% |
| Chemical Engineering | 2 | 3% |
| Other | 8 | 12% |
| Unknown | 14 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 March 2014.
All research outputs
#5,089,278
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#1,221
of 8,034 outputs
Outputs of similar age
#12,658
of 67,945 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#23
of 86 outputs
Altmetric has tracked 24,119,703 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,034 research outputs from this source. They receive a mean Attention Score of 4.3. This one has gotten more attention than average, scoring higher than 72% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 67,945 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 86 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 54% of its contemporaries.