| Title |
The state of the art in the analysis of two-dimensional gel electrophoresis images
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, August 2007
|
| DOI | 10.1007/s00253-007-1128-0 |
| Pubmed ID | |
| Authors |
Matthias Berth, Frank Michael Moser, Markus Kolbe, Jörg Bernhardt |
| Abstract |
Software-based image analysis is a crucial step in the biological interpretation of two-dimensional gel electrophoresis experiments. Recent significant advances in image processing methods combined with powerful computing hardware have enabled the routine analysis of large experiments. We cover the process starting with the imaging of 2-D gels, quantitation of spots, creation of expression profiles to statistical expression analysis followed by the presentation of results. Challenges for analysis software as well as good practices are highlighted. We emphasize image warping and related methods that are able to overcome the difficulties that are due to varying migration positions of spots between gels. Spot detection, quantitation, normalization, and the creation of expression profiles are described in detail. The recent development of consensus spot patterns and complete expression profiles enables one to take full advantage of statistical methods for expression analysis that are well established for the analysis of DNA microarray experiments. We close with an overview of visualization and presentation methods (proteome maps) and current challenges in the field. |
Mendeley readers
The data shown below were compiled from readership statistics for 289 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 6 | 2% |
| United States | 3 | 1% |
| Portugal | 2 | <1% |
| Poland | 2 | <1% |
| United Kingdom | 2 | <1% |
| Chile | 1 | <1% |
| Czechia | 1 | <1% |
| Sweden | 1 | <1% |
| France | 1 | <1% |
| Other | 4 | 1% |
| Unknown | 266 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 69 | 24% |
| Student > Ph. D. Student | 59 | 20% |
| Student > Master | 38 | 13% |
| Student > Bachelor | 28 | 10% |
| Student > Doctoral Student | 15 | 5% |
| Other | 55 | 19% |
| Unknown | 25 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 146 | 51% |
| Biochemistry, Genetics and Molecular Biology | 34 | 12% |
| Medicine and Dentistry | 16 | 6% |
| Engineering | 16 | 6% |
| Chemistry | 11 | 4% |
| Other | 33 | 11% |
| Unknown | 33 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 August 2020.
All research outputs
#2,671,203
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#262
of 8,034 outputs
Outputs of similar age
#5,906
of 69,596 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#6
of 64 outputs
Altmetric has tracked 24,119,703 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,034 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 69,596 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 64 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 89% of its contemporaries.