| Title |
A Simple Sonication Improves Protein Signal in Matrix-Assisted Laser Desorption Ionization Imaging
|
|---|---|
| Published in |
Journal of the American Society for Mass Spectrometry, February 2018
|
| DOI | 10.1007/s13361-018-1889-y |
| Pubmed ID | |
| Authors |
Li-En Lin, Pin-Rui Su, Hsin-Yi Wu, Cheng-Chih Hsu |
| Abstract |
Proper matrix application is crucial in obtaining high quality matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI). Solvent-free sublimation was essentially introduced as an approach of homogeneous coating that gives small crystal size of the organic matrix. However, sublimation has lower extraction efficiency of analytes. Here, we present that a simple sonication step after the hydration in standard sublimation protocol significantly enhances the sensitivity of MALDI MSI. This modified procedure uses a common laboratory ultrasonicator to immobilize the analytes from tissue sections without noticeable delocalization. Improved imaging quality with additional peaks above 10 kDa in the spectra was thus obtained upon sonication treatment. Graphical Abstract ᅟ. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 17 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 18% |
| Student > Ph. D. Student | 2 | 12% |
| Student > Doctoral Student | 1 | 6% |
| Student > Bachelor | 1 | 6% |
| Other | 1 | 6% |
| Other | 3 | 18% |
| Unknown | 6 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 3 | 18% |
| Medicine and Dentistry | 2 | 12% |
| Biochemistry, Genetics and Molecular Biology | 2 | 12% |
| Chemical Engineering | 1 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 7 | 41% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 February 2018.
All research outputs
#20,663,600
of 25,382,440 outputs
Outputs from Journal of the American Society for Mass Spectrometry
#3,086
of 3,835 outputs
Outputs of similar age
#343,060
of 448,849 outputs
Outputs of similar age from Journal of the American Society for Mass Spectrometry
#41
of 58 outputs
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We're also able to compare this research output to 58 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.