| Title |
The Effect of the lacY Gene on the Induction of IPTG Inducible Promoters, Studied in Escherichia coli and Pseudomonas fluorescens
|
|---|---|
| Published in |
Current Microbiology, June 1998
|
| DOI | 10.1007/s002849900320 |
| Pubmed ID | |
| Authors |
Lars H. Hansen, Steen Knudsen, Søren J. Sørensen |
| Abstract |
The role of the Escherichia coli lacY gene product (the lactose permease) in the induction of isopropyl-beta-D-thiogalactopyranoside (IPTG) inducible promoters was studied in E. coli and P. fluorescens. This was done by comparing strains containing a lacIPOZYA chromosomal insert with newly constructed strains containing inserts without the lacY gene (lacIPOZ). The lactose operon inserts were introduced as single-copy chromosomal inserts to eliminate differences in expression caused by differences in copy number. Comparison between the two types of inserts showed that the lactose permease was essential to allow growth on lactose by both bacteria and that the lactose permease plays an important role in transporting the inducer IPTG across the membrane of P. fluorescens. The use of a functional lactose permease allows expression of beta-galactosidase to increase more than fivefold from a wild-type lac promoter in P. fluorescens SS1001. We suggest that an increase in the rate of protein synthesis from lac-type promoters could be enhanced if an active lactose permease is present as well. |
Mendeley readers
The data shown below were compiled from readership statistics for 499 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | <1% |
| Denmark | 2 | <1% |
| Finland | 1 | <1% |
| United Kingdom | 1 | <1% |
| Canada | 1 | <1% |
| Germany | 1 | <1% |
| Belgium | 1 | <1% |
| Chile | 1 | <1% |
| Romania | 1 | <1% |
| Other | 1 | <1% |
| Unknown | 485 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 185 | 37% |
| Student > Master | 67 | 13% |
| Student > Ph. D. Student | 65 | 13% |
| Researcher | 33 | 7% |
| Other | 13 | 3% |
| Other | 25 | 5% |
| Unknown | 111 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 142 | 28% |
| Biochemistry, Genetics and Molecular Biology | 124 | 25% |
| Chemistry | 30 | 6% |
| Engineering | 26 | 5% |
| Medicine and Dentistry | 18 | 4% |
| Other | 44 | 9% |
| Unknown | 115 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 October 2023.
All research outputs
#4,863,838
of 23,420,064 outputs
Outputs from Current Microbiology
#204
of 2,461 outputs
Outputs of similar age
#4,624
of 34,593 outputs
Outputs of similar age from Current Microbiology
#1
of 6 outputs
Altmetric has tracked 23,420,064 research outputs across all sources so far. Compared to these this one has done well and is in the 76th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,461 research outputs from this source. They receive a mean Attention Score of 3.2. This one has done well, scoring higher than 85% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 34,593 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them