| Title |
Anti-Oxidative Effects of Melatonin Receptor Agonist and Omega-3 Polyunsaturated Fatty Acids in Neuronal SH-SY5Y Cells: Deciphering Synergic Effects on Anti-Depressant Mechanisms
|
|---|---|
| Published in |
Molecular Neurobiology, February 2018
|
| DOI | 10.1007/s12035-018-0899-x |
| Pubmed ID | |
| Authors |
Senthil Kumaran Satyanarayanan, Yin-Hwa Shih, Yu-Chuan Chien, Shih-Yi Huang, Piotr Gałecki, Siegfried Kasper, Jane Pei-Chen Chang, Kuan-Pin Su |
| Abstract |
Omega-3 polyunsaturated fatty acids (n-3 or omega-3 PUFAs) and melatonin receptor agonist ramelteon (RMT) both display antidepressant effects, while their cellular effects on anti-oxidative and neuroprotective mechanisms might be different. In this study, we aimed to decipher the individual and synergistic actions of n-3 PUFAs and RMT, as compared with the conventional antidepressant fluoxetine (FLX), in a cellular model of oxidative stress, which might play an important role in the pathophysiology of depression and associated disorders. We investigated the rescue and prevention effects of FLX, RMT, and n-3 PUFAs, e.g., eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), by using cell viability in SH-SY5Y cells under oxidative stress along with measurements of key cellular markers of oxidative stress, inflammatory, and neuroprotection. The results revealed that the RMT and EPA combination significantly increased the cell viability in a dose-dependent manner. RMT showed preventive effects, FLX and DHA possessed rescue effects, while EPA showed both rescue and preventive effects. We observed the dose-dependent activation and translocation of nuclear factor-κB to the nucleus augmented by the expressions of peroxisome proliferator activator receptor-gamma, tyrosine hydroxylase, c-Fos expression, and reactive oxygen species, implying that RMT and EPA combination reversed oxidative and neuroinflammatory pathophysiology and protected the neuronal cells from further damage. The results demonstrated that RMT and EPA synergistically provide effective neuroprotective, anti-oxidative/inflammatory effect against oxidative stress. Our study provides pre-clinical evidence to conduct future clinical trials of using n-3 PUFAs/RMT combination in depressive disorders. |
X Demographics
The data shown below were collected from the profiles of 22 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Norway | 3 | 14% |
| Spain | 3 | 14% |
| Portugal | 1 | 5% |
| Venezuela, Bolivarian Republic of | 1 | 5% |
| United States | 1 | 5% |
| Unknown | 13 | 59% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 20 | 91% |
| Practitioners (doctors, other healthcare professionals) | 2 | 9% |
Mendeley readers
The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 32 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 9 | 28% |
| Professor | 4 | 13% |
| Researcher | 3 | 9% |
| Other | 2 | 6% |
| Student > Doctoral Student | 2 | 6% |
| Other | 5 | 16% |
| Unknown | 7 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 7 | 22% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 13% |
| Neuroscience | 3 | 9% |
| Biochemistry, Genetics and Molecular Biology | 2 | 6% |
| Psychology | 2 | 6% |
| Other | 4 | 13% |
| Unknown | 10 | 31% |
Attention Score in Context
This research output has an Altmetric Attention Score of 13. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 March 2023.
All research outputs
#2,801,488
of 25,373,627 outputs
Outputs from Molecular Neurobiology
#337
of 3,959 outputs
Outputs of similar age
#62,508
of 447,342 outputs
Outputs of similar age from Molecular Neurobiology
#16
of 120 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,959 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one has done particularly well, scoring higher than 91% of its peers.
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We're also able to compare this research output to 120 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 87% of its contemporaries.